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. 2023 Feb 3;8(2):101.
doi: 10.3390/tropicalmed8020101.

Rotavirus Infection and Genotyping in Yantai, Shandong Province, 2017-2019

Zhenlu Sun  1   2 , Guifang Zhang  1 , Chunyan Li  1 , Peihua Niu  2 , Xia Li  1 , Qiao Gao  1 , Kai Guo  1 , Ruiqing Zhang  2 , Ji Wang  2 , Xuejun Ma  2   3
Affiliations

Rotavirus Infection and Genotyping in Yantai, Shandong Province, 2017-2019

Zhenlu Sun et al. Trop Med Infect Dis. .

Abstract

Purpose: Rotavirus (RV) ranked first among infectious diarrhea-causing pathogens in Yantai from 2017 to 2019. This study investigated the seroserotypes of RV in Yantai, Shandong, from 2017 to 2019 to identify the dominant serotypes and explore the epidemic pattern, aiming to effectively reduce the infection rate, better guide vaccination, and help in epidemiological prevention and control.

Methods: A total of 2227 human diarrhea samples were collected from 2017 to 2019 in Yantai. The VP7 (G serotype) and VP4 (P serotype) genes of 467 RV-positive samples were amplified using two-round nested reverse transcription-polymerase chain reaction for G/P genotyping.

Results: The genotyping results of RV in Yantai from 2017 to 2019 revealed that G9 was the dominant serotype for all G serotypes, P[8] was the dominant serotype for all P serotypes, and G9P[8] was the dominant serotype for all G/P combinations. G9 serotype accounted for 60.84%, 95.65%, and 83.76% of the total RV samples collected in 2017, 2018, and 2019, respectively. P[8] accounted for 75.52%, 94.69%, and 88.89% of the RV-positive samples collected in 2017, 2018, and 2019, respectively. G9P[8] accounted for 60.84%, 94.69%, and 83.76% of the total RV samples collected in 2017, 2018, and 2019, respectively. Of the total 467 samples from 2017 to 2019, G2P[4] accounted for 3.64% (17/467), G3P[8] for 1.28% (6/467), and G1P[8] for 0.86% (4/467).

Conclusion: This study revealed the epidemiological characteristics of RV infection and the development pattern of dominant serotypes in Yantai in recent years, guiding the selection of RV vaccines. The prioritization of vaccines containing G9 serotype for infants in Yantai in recent years is recommended.

Keywords: diarrhea; genotyping; infection; rotavirus; serotypes; vaccine.

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Conflict of interest statement

All the authors declare that no competing interest exist.

Figures

Figure 1
Figure 1
Electrophoretic results of Rotavirus G genotyping in some samples from 2017 to 2019.
Figure 2
Figure 2
Electrophoretic results of Rotavirus P genotyping in some samples from 2017 to 2019.
Figure 3
Figure 3
Composition ratio of rotavirus genoserotypes in Yantai in 2017–2019.
See this image and copyright information in PMC

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References

    1. Estes M.K., Greenberg H.B. Rotaviruses. Wolters Kluwer Health/Lippincott Williams and Wilkins; Philadelphia, PA, USA: 2013.
    1. Chen J.Z., Settembre E.C., Aoki S.T., Zhang X., Bellamy A.R., Dormitzer P.R., Harrison S.C., Grigorieff N. Molecular interactions in rotavirus assembly and uncoating seen by high-resolution cryo-EM. Proc. Natl. Acad. Sci. USA. 2009;106:10644–10648. doi: 10.1073/pnas.0904024106. - DOI - PMC - PubMed
    1. Li Z., Baker M.L., Jiang W., Estes M.K., Prasad B.V. Rotavirus architecture at subnanometer resolution. J. Virol. 2009;83:1754–1766. doi: 10.1128/JVI.01855-08. - DOI - PMC - PubMed
    1. Settembre E.C., Chen J.Z., Dormitzer P.R., Grigorieff N., Harrison S.C. Atomic model of an infectious rotavirus particle. EMBO J. 2011;30:408–416. doi: 10.1038/emboj.2010.322. - DOI - PMC - PubMed
    1. Kirkwood C.D. Genetic and antigenic diversity of human rotaviruses: Potential impact on vaccination programs. J. Infect. Dis. 2010;202:S43–S48. doi: 10.1086/653548. - DOI - PubMed

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