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. 2023 Feb 8;12(2):354.
doi: 10.3390/antibiotics12020354.

Evaluation of Antiproliferative, Antimicrobial, Antioxidant, Antidiabetic and Phytochemical Analysis of Anogeissus dhofarica A. J. Scott

Rabia Maqsood  1   2 , Faizullah Khan  1   3 , Saeed Ullah  1 , Ajmal Khan  1 , Habib Al-Jahdhami  1 , Javid Hussain  4 , Afaf M Weli  5 , Danial Maqsood  6 , Shaikh Mizanoor Rahman  1 , Amjad Hussain  2 , Najeeb Ur Rehman  1 , Ahmed Al-Harrasi  1
Affiliations

Evaluation of Antiproliferative, Antimicrobial, Antioxidant, Antidiabetic and Phytochemical Analysis of Anogeissus dhofarica A. J. Scott

Rabia Maqsood et al. Antibiotics (Basel). .

Abstract

In the current study, methanol (ADAM) extracts and their fractions, including chloroform (ADAC), ethyl acetate (ADAE), n-hexane (ADAH), and aqueous (ADAA) fractions, were prepared from aerial parts of Anogeissus dhofarica and evaluated for phytochemical assessment, high-resolution electrospray ionization mass spectrometry (HR-ESI-MS) analysis, and in vitro bioassays. The qualitative analysis determined that, except alkaloids, all the representative groups were found to be present in the analyzed samples. Samples under quantitative study displayed the highest amount of total phenolic contents in the ADAE fraction, while total flavonoid contents were highest in the ADAM extract. The ADAM extract was subjected to HR-ESI-MS to identify the chemical constituents that presented twenty-two bioactive ingredients, outlined for the first time from A. dhofarica, mainly contributed by sub-class flavanones. In the case of antimicrobial activity, the ADAE extract revealed an effective zone of inhibition (ZOI) against the Gram-positive bacterial strain (Staphylococcus aureus) with an MIC value of 0.78 ± 0.3 mg/mL, while the ADAA extract exhibited higher ZOI (34 ± 0.12 mm) against the fungal strain Candida kruzei with an MIC of 0.78 mg/mL. In the DPPH (2,2-diphenyl-1-picrylhydrazyl) analysis, the ADAE extract exhibited a maximum scavenging potential with an IC50 of 9.8 ± 1.2 μg/mL, succeeded by the ADAM extract with an IC50 of 17.4 ± 0.4 μg/mL free radical scavenging capability. In the antidiabetic assessment, the ADAE extract was the most effective, with an IC50 of 6.40 ± 0.1 μg/mL, while the same extract demonstrated prominent activity with 30.8% viability and an IC50 of 6.2 ± 0.3 μg/mL against breast cancer cell lines. The brine shrimp lethality assay demonstrated a correlation with the in vitro cytotoxicity assay, showing the ADAE extract as the most active, with a 70% mortality rate and an LC50 of 300.1 μg/mL. In conclusion, all the tested samples, especially the ADAE and ADAM extracts, have significant capabilities for the investigated activities that could be due to the presence of the bioactive compounds.

Keywords: Anogeissus dhofarica A. J. Scott; HR-ESI-MS; antidiabetic; antimicrobial; antioxidant; cytotoxicity; phytochemical analysis.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Scheme 1
Scheme 1
General fractionation scheme for the solvent-solvent extraction of A. dhofarica.
Figure 1
Figure 1
Structures of the tentatively identified compounds in ethyl acetate (ADAE) and methanol (ADAM) extracts of A. dhofarica.
Figure 2
Figure 2
Comparison of LC50 values of different polarity extracts of A. dhofarica with positive (KMNO4) and negative (DMSO) controls.
See this image and copyright information in PMC

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References

    1. Raina H., Soni G., Jauhari N., Sharma N., Bharadvaja N. Phytochemical importance of medicinal plants as potential sources of anticancer agents. Turk. J. Bot. 2014;38:1027–1035. doi: 10.3906/bot-1405-93. - DOI
    1. Mohammed A.H. Importance of medicinal plants. Res. Pharm. Health Sci. 2019;5:124–125. doi: 10.32463/rphs.2019.v05i02.01. - DOI
    1. Muhammad Z., Nasir R., Asim M., Fozia A., Munawar I., Muhammad M., Muhammad S. Antioxidant, antibacterial, antifungal activities and phytochemical analysis of dagger (Yucca aloifolia) leaves extracts. J. Med. Plants Res. 2013;7:243–249.
    1. Ur Rehman F., Kalsoom M., Adnan M., Fazeli-Nasab B., Naz N., Ilahi H., Ali M.F., Ilyas M., Yousaf G., Toor M.D. Importance of medicinal plants in human and plant pathology: A review. Int. J. Pharm. Biomed. Res. 2021;8:1–11. doi: 10.18782/2394-3726.1110. - DOI
    1. Ullah A., Munir S., Badshah S.L., Khan N., Ghani L., Poulson B.G., Emwas A.-H., Jaremko M. Important flavonoids and their role as a therapeutic agent. Molecules. 2020;25:5243. doi: 10.3390/molecules25225243. - DOI - PMC - PubMed

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