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. 2023 Feb 10;12(2):370.
doi: 10.3390/antibiotics12020370.

Prevalence of Antimicrobial Resistance and Clonal Relationship in ESBL/AmpC-Producing Proteus mirabilis Isolated from Meat Products and Community-Acquired Urinary Tract Infection (UTI-CA) in Southern Brazil

Matheus Silva Sanches  1 , Luana Carvalho Silva  1 , Caroline Rodrigues da Silva  2 , Victor Hugo Montini  1 , Bruno Henrique Dias de Oliva  1 , Gustavo Henrique Migliorini Guidone  1 , Mara Corrêa Lelles Nogueira  2 , Maísa Fabiana Menck-Costa  3 , Renata Katsuko Takayama Kobayashi  3 , Eliana Carolina Vespero  4 , Sergio Paulo Dejato Rocha  1
Affiliations

Prevalence of Antimicrobial Resistance and Clonal Relationship in ESBL/AmpC-Producing Proteus mirabilis Isolated from Meat Products and Community-Acquired Urinary Tract Infection (UTI-CA) in Southern Brazil

Matheus Silva Sanches et al. Antibiotics (Basel). .

Abstract

The present study aimed to evaluate the prevalence of antimicrobial resistance and clonal relationships in Proteus mirabilis isolated from chicken meat, beef, pork, and community-acquired urinary tract infections (UTI-CA). Chicken meat isolates showed the highest multidrug resistance (MDR), followed by those from pork and UTI-CA, whereas beef had relatively few MDR strains. All sources had strains that carried blaCTX-M-65, whereas blaCTX-M-2 and blaCMY-2 were only detected in chicken meat and UTI-CA isolates. This indicates that chicken meat should be considered an important risk factor for the spread of P. mirabilis carrying ESBL and AmpC. Furthermore, ESBL/AmpC producing strains were resistant to a greater number of antimicrobials and possessed more resistance genes than non-producing strains. In addition, the antimicrobial resistance genes qnrD, aac(6')-Ib-cr, sul1, sul2, fosA3, cmlA, and floR were also found. Molecular typing showed a genetic similarity between chicken meat and UTI-CA isolates, including some strains with 100% similarity, indicating that chicken can be a source of P. mirabilis causing UTI-CA. It was concluded that meat, especially chicken meat, can be an important source of dissemination of multidrug-resistant P. mirabilis in the community.

Keywords: Proteus mirabilis; bla CMY-2; bla CTX-M-2; bla CTX-M-65; multidrug resistance.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Comparison of the prevalence (%) of antimicrobial resistance in P. mirabilis isolated from chicken (n = 200), pork (n = 83), beef (n = 100), and UTI-CA (n = 200).
Figure 2
Figure 2
Antimicrobial resistance exhibited by ESBL-producing and non-ESBL-producing P. mirabilis strains isolated from chicken, pork, beef, and UTI-CA. * p < 0.05 by Fisher test.
Figure 3
Figure 3
Antimicrobial resistance exhibited by AmpC-producing and non-AmpC-producing P. mirabilis strains isolated from chicken, pork, beef, and UTI-CA. * p < 0.05 by Fisher test.
Figure 4
Figure 4
Dendrogram obtained from Not I-PFGE typing of the 87 P. mirabilis ESBL-AmpC producers. Dendrogram was constructed using optimization 0% and tolerance 1.5%. SXT: sulfamethoxazole-trimethoprim; AMC: amoxicillin and clavulanate; AMP: ampicillin; CEF: cephalothin; FOX: cefoxitin; CRO: ceftriaxone; CAZ: ceftazidime; CFT: ceftiofur; CPM: cefepime; ATM: aztreonam; NAL: nalidixic acid; ENO: enrofloxacin; CIP: ciprofloxacin; NOR: norfloxacin; CHL: chloramphenicol; FFC: florfenicol; GEN: gentamicin; TOB: tobramycin; FOS: fosfomycin; AMI: amikacin; ETP: ertapenem; C.P: collect point.
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