Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Feb 9;13(2):250.
doi: 10.3390/bios13020250.

Single Printing Step Prussian Blue Bulk-Modified Transducers for Oxidase-Based Biosensors

Darya Vokhmyanina  1 , Elena Daboss  1 , Olesya Sharapova  1 , Mariia Mogilnikova  1 , Arkady Karyakin  1
Affiliations

Single Printing Step Prussian Blue Bulk-Modified Transducers for Oxidase-Based Biosensors

Darya Vokhmyanina et al. Biosensors (Basel). .

Abstract

We report on hydrogen peroxide sensors made through a single printing step with carbon ink containing catalytically synthesized Prussian blue nanoparticles. Despite their reduced sensitivity, the resulting bulk-modified sensors displayed both a wider linear calibration range (5 × 10-7-1 × 10-3 M) and an approximately four times lower detection limit versus the surface-modified sensors due to the dramatically decreased noise resulting in, on average, a six times higher signal-to-noise ratio. The corresponding glucose and lactate biosensors demonstrated similar and even higher sensitivities compared to those of biosensors based on surface-modified transducers. The biosensors have been validated through analysis of human serum. The decreased time and cost for production of single printing step bulk-modified transducers, as well as their analytical performance characteristics, which are advantageous over conventional surface-modified ones, would be expected to enable their wide use in (bio)sensorics.

Keywords: Prussian blue nanoparticles; biosensors; glucose; hydrogen peroxide; lactate; screen-printed electrodes.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
The example of hydrodynamic size distribution obtained by dynamic light scattering and fit to the log-normal distribution for Prussian Blue nanoparticles with the average diameter of 35 nm.
Figure 2
Figure 2
Energy dispersive X-ray (EDX) spectra of a PB nanoparticle-modified carbon screen-printed electrode (Prussian blue content 2.15 mg/g).
Figure 3
Figure 3
(a) The background and noise level for surface-modified blank electrodes (black line) and electrodes with printed Prussian blue nanoparticles (PBNP) (red line and inset); (b) signal-to-noise ratio dependance on Prussian blue nanoparticle concentration in carbon/graphite paste.
Figure 4
Figure 4
(a) The calibration curve for the printed hydrogen peroxide sensor with 2.15 mg/g of PBNPs in carbon/graphite ink; (b) sensitivity dependance on Prussian blue nanoparticle concentration in carbon/graphite ink.
Figure 5
Figure 5
The construction scheme and functioning of the biosensor system made through the single printing step of carbon ink containing PBNPs.
Figure 6
Figure 6
Sensitivity dependance on Prussian blue nanoparticle concentration in carbon/graphite ink for (a) glucose and (b) lactate biosensors based on printed hydrogen peroxide sensors.
See this image and copyright information in PMC

References

    1. Niethammer P., Grabher C., Look A.T., Mitchison T.J. A tissue-scale gradient of hydrogen peroxide mediates rapid wound detection in zebrafish. Nature. 2009;459:996–999. doi: 10.1038/nature08119. - DOI - PMC - PubMed
    1. Chen W., Cai S., Ren Q.Q., Wen W., Zhao Y.D. Recent advances in electrochemical sensing for hydrogen peroxide: A review. Analyst. 2012;137:49–58. doi: 10.1039/C1AN15738H. - DOI - PubMed
    1. Hawe A., Wiggenhorn M., van de Weert M., Garbe J.H., Mahler H.-C., Jiskoot W. Forced Degradation of Therapeutic Proteins. J. Pharm. Sci. 2012;101:895–913. doi: 10.1002/jps.22812. - DOI - PubMed
    1. Seresirikachorn K., Phoophiboon V., Chobarporn T., Tiankanon K., Aeumjaturapat S., Chusakul S., Snidvongs K. Decontamination and reuse of surgical masks and N95 filtering facepiece respirators during the COVID-19 pandemic: A systematic review. Infect. Control Hosp. Epidemiol. 2021;42:25–30. doi: 10.1017/ice.2020.379. - DOI - PMC - PubMed
    1. Kumkrong P., Scoles L., Brunet Y., Baker S. Determination of hydrogen peroxide on N95 masks after sanitization using a colorimetric method. Methodsx. 2021;8:101485. doi: 10.1016/j.mex.2021.101485. - DOI - PMC - PubMed

LinkOut - more resources