Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Feb 9;14(2):441.
doi: 10.3390/genes14020441.

Serum MicroRNAs as Predictors for HCV Progression and Response to Treatment in Pakistani Patients

Sadia Manzoor  1 , Imran Riaz Malik  1 , Shah Jahan  2 , Muhammad Bilal Sarwar  3 , Asma Bashir  4 , Sulaiman Shams  5 , Abrar Hussain  6
Affiliations

Serum MicroRNAs as Predictors for HCV Progression and Response to Treatment in Pakistani Patients

Sadia Manzoor et al. Genes (Basel). .

Abstract

Hepatitis is one of the common liver diseases, imposing a heavy health burden worldwide. Acute hepatitis may develop into chronic hepatitis, progressing to cirrhosis and hepatocellular carcinoma. In the present study, the expression of miRNAs was quantified by real-time PCR, such as miRNA-182, 122, 21, 150, 199, and 222. Along with the control group, HCV was divided into chronic, cirrhosis, and HCC groups. The treated group was also included after the successful treatment of HCV. Biochemical parameters, such as ALT, AST, ALP, bilirubin, viral load, and AFP (HCC), were also evaluated in all of the study groups. We compared the control and diseased groups; these parameters showed significant results (p = 0.000). The viral load was high in HCV but was not detected after treatment. miRNA-182 and miRNA-21 were overexpressed with disease progression, while the expression of miRNA-122 and miRNA-199 was increased compared with the control, but decreased in the cirrhosis stage compared with chronic and HCC. The expression of miRNA-150 was increased in all of the diseased groups compared with the control, but decreased compared with the chronic group. We compared the chronic and treated groups and then all of these miRNAs were down-regulated after treatment. These microRNAs could be used as potential biomarkers for diagnosing different stages of HCV.

Keywords: HCC; biomarker; chronic; cirrhosis; hepatitis C; treatment.

PubMed Disclaimer

Conflict of interest statement

The authors declare no conflict of interest relevant to this study.

Figures

Figure 1
Figure 1
Graphical representation of comparison of viral load among males and females.
Figure 2
Figure 2
Graphical representation of analysis of variance in viral load level among the chronic, cirrhosis, HCC, treated, and control groups.
Figure 3
Figure 3
Graphical representation of the comparison of viral load among the chronic and treated groups.
Figure 4
Figure 4
Graphical representation of expression analysis of miRNAs in diseased and control groups using real-time PCR.
Figure 5
Figure 5
Graphical representation of the expression analysis of miRNAs in the diseased and treated groups using real-time PCR.
See this image and copyright information in PMC

References

    1. Chen G., Cheung R., Tom J.W. Hepatitis: Sedation and anesthesia implications. Anesth. Prog. 2017;64:106–118. doi: 10.2344/anpr-64-02-13. - DOI - PMC - PubMed
    1. Qu C., Zhang S., Li Y., Wang Y., Peppelenbosch M.P., Pan Q. Mitochondria in the biology, pathogenesis, and treatment of hepatitis virus infections. Rev. Med. Virol. 2019;29:e2075. doi: 10.1002/rmv.2075. - DOI - PMC - PubMed
    1. Dubuisson J., Cosset F.L. Virology and cell biology of the hepatitis C virus life cycle–An update. J. Hepatol. 2014;61:S3–S13. doi: 10.1016/j.jhep.2014.06.031. - DOI - PubMed
    1. Warkad S.D., Nimse S.B., Song K.S., Kim T. HCV detection, discrimination, and genotyping technologies. Sensors. 2018;18:3423. doi: 10.3390/s18103423. - DOI - PMC - PubMed
    1. Page K., Hahn J.A., Evans J., Shiboski S., Lum P., Delwart E. Acute hepatitis C virus infection in young adult injection drug users: A prospective study of incident infection, resolution, and reinfection. J. Infect. Dis. 2009;200:1216–1226. doi: 10.1086/605947. - DOI - PMC - PubMed