[Comparison of the effectiveness of the interaction of ribo- and deoxyriboprimers with DNA-polymerase from the human placenta]

Abstract

The Km and Vmax values for primers d(pA)n, d(pT)n, r(pA)n, r(pU)n where n = 1-16, were compared. The Km values for minimal primers dTMP, dAMP, rUMP, rAMP were found to be 48, 71, 602 and 602 microM, respectively. The Vmax value for any NMP made up approximately 7% of that for (pN)10. The lengthening of any primer per one mononucleotide unit for n from 1 to 10 resulted in the decrease of the Km value 1.8-fold and the increase of the Vmax value 1.35-fold. The ratios of the Km values for primers r(pA)n-d(pA)n and r(pU)n-d(pT)n were 7.5 and 12.5, respectively, for any n. The Km value for [d[pT)8]r(pU) primer was the same as for r(pU)9, but not for d(pT)9. Decanucleotide [d(Tp)9]ddT interacted with the polymerase competitively to the template, but not to the primer. The primer's 3'-OH group was supposed to form the hydrogen bond with the enzyme. The absence of 3'-hydroxygroup in [d(Tp)9]ddT resulted in its inability to compete effectively with the primer. The difference of the affinity of ribo- and deoxyriboprimers is due, apparently, to the existence of the different conformation of the furanose rings in the ribose and deoxyribose.