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. 2023 Jan 26;11(2):315.
doi: 10.3390/microorganisms11020315.

Antibiotic Susceptibility and Molecular Typing of Invasive Haemophilus influenzae Isolates, with Emergence of Ciprofloxacin Resistance, 2017-2021, Italy

Maria Giufrè  1 , Rita Cardines  1 , Manuela Marra  2 , Maria Carollo  2 , Marina Cerquetti  1 , Paola Stefanelli  1
Affiliations

Antibiotic Susceptibility and Molecular Typing of Invasive Haemophilus influenzae Isolates, with Emergence of Ciprofloxacin Resistance, 2017-2021, Italy

Maria Giufrè et al. Microorganisms. .

Abstract

Haemophilus influenzae invasive disease is a severe infection that needs rapid antibiotic therapy. The aim of the study was to perform and evaluate the serotype distribution, antibiotic susceptibility and molecular characteristics of 392 H. influenzae invasive isolates collected during 2017-2021 in Italy. The majority of isolates were NTHi (305/392, 77.8%), followed by Hib (49/392, 12.5%). Ampicillin resistance was frequently detected (85/392, 21.7%): 12.2% were β-lactamase producers (all blaTEM except one blaROB), 9.4% were β-lactamase-negative ampicillin-resistant (BLNAR), with mutations in the ftsI gene. Six isolates were resistant to ciprofloxacin, with substitutions in GyrA and ParC. An MLST analysis revealed the occurrence of international resistant clones, such as ST103 and ST14, highlighting the importance of molecular surveillance.

Keywords: Haemophilus influenzae; MLST; antibiotic resistance; quinolone resistance; surveillance; whole genome sequencing.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Minimum spanning tree based on MLST alleles showing genetic relationships among STs of 176 H. influenzae isolates from invasive disease, Italy, 2017–2021. Each node represents a different ST with the size of nodes proportional to the number of included isolates. ST number is indicated in each node. Capsular type is indicated in different colors, as shown in the figure.
See this image and copyright information in PMC

References

    1. Bogaert D., Keijser B., Huse S., Rossen J., Veenhoven R., van Gils E., Bruin J., Montijn R., Bonten M., Sanders E. Variability and diversity of nasopharyngeal microbiota in children: A metagenomics analysis. PLoS ONE. 2011;6:e17035. doi: 10.1371/journal.pone.0017035. - DOI - PMC - PubMed
    1. Lemon K.P., Klepac-Ceraj V., Schiffer H.K., Brodie E.L., Lynch S.V., Kolter R. Comparative analyses of the bacterial microbiota of the human nostril and oropharynx. Mbio. 2010;1:e00129-10. doi: 10.1128/mBio.00129-10. - DOI - PMC - PubMed
    1. Turk D.C. The pathogenicity of Haemophilus influenzae. J. Med. Microbiol. 1984;18:1–16. doi: 10.1099/00222615-18-1-1. - DOI - PubMed
    1. Peltola H. Worldwide Haemophilus influenzae type b disease at the beginning of the 21st century: Global analysis of the disease burden 25 years after the use of the polysaccharide vaccine and a decade after the advent of conjugates. Clin. Microbiol. Rev. 2000;13:302–317. doi: 10.1128/CMR.13.2.302. - DOI - PMC - PubMed
    1. European Centre for Disease Prevention and Control (ECDC) Haemophilus influenzae Annual Epidemiological Report for 2018. European Centre for Disease Prevention and Control (ECDC); Stockholm, Sweden: 2020.

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