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Review
. 2023 Feb 17;11(2):510.
doi: 10.3390/microorganisms11020510.

The Recent Advances in the Utility of Microbial Lipases: A Review

Sajid Ali  1 , Sumera Afzal Khan  2 , Muhammad Hamayun  3 , In-Jung Lee  4
Affiliations
Review

The Recent Advances in the Utility of Microbial Lipases: A Review

Sajid Ali et al. Microorganisms. .

Abstract

Lipases are versatile biocatalysts and are used in different bioconversion reactions. Microbial lipases are currently attracting a great amount of attention due to the rapid advancement of enzyme technology and its practical application in a variety of industrial processes. The current review provides updated information on the different sources of microbial lipases, such as fungi, bacteria, and yeast, their classical and modern purification techniques, including precipitation and chromatographic separation, the immunopurification technique, the reversed micellar system, aqueous two-phase system (ATPS), aqueous two-phase flotation (ATPF), and the use of microbial lipases in different industries, e.g., the food, textile, leather, cosmetics, paper, and detergent industries. Furthermore, the article provides a critical analysis of lipase-producing microbes, distinguished from the previously published reviews, and illustrates the use of lipases in biosensors, biodiesel production, and tea processing, and their role in bioremediation and racemization.

Keywords: biocatalysis; industrial application; lipase engineering; microbial lipase; purification.

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Conflict of interest statement

All authors declare that they have no conflict of interest.

Figures

Figure 1
Figure 1
A representative diagram of an antibody used for the immunopurification of microbial lipases.
Figure 2
Figure 2
A schematic diagram of the two-step extraction in/with micellar systems.
Figure 3
Figure 3
Schematic presentation of an aqueous two-phase system (ATPS) for lipase purification.
Figure 4
Figure 4
Schematic presentation of aqueous two-phase flotation (ATPF) for lipase purification.
See this image and copyright information in PMC

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