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. 2023 Feb 18;11(2):522.
doi: 10.3390/microorganisms11020522.

Bioremediation of Oil-Contaminated Soil of the Republic of Kazakhstan Using a New Biopreparation

Tatiana Vyacheslavovna Funtikova  1 , Lenar Imametdinovich Akhmetov  1 , Irina Filippovna Puntus  1 , Pavel Alexeevich Mikhailov  1 , Nurbol Orynbasaruly Appazov  2 , Roza Abdibekovna Narmanova  2 , Andrey Evgenievich Filonov  1 , Inna Petrovna Solyanikova  1
Affiliations

Bioremediation of Oil-Contaminated Soil of the Republic of Kazakhstan Using a New Biopreparation

Tatiana Vyacheslavovna Funtikova et al. Microorganisms. .

Abstract

A new biopreparation is developed to clean soils from oil pollution in the arid climate of the Republic of Kazakhstan. The biopreparation includes bacterial strains R. qingshengii F2-1, R. qingshengii F2-2, and P. alloputida BS3701. When using the biopreparation in a liquid mineral medium with 15% crude oil, laboratory studies have revealed degradation of 48% n-alkanes and 39% of PAHs after 50 days. The effectiveness of the biopreparation has been demonstrated in field experiments in the soil contaminated with 10% crude oil at the K-Kurylys landfill, Republic of Kazakhstan. During the six-month field experiment, the number of oil degraders reached 107 CFU/g soil, which degraded 70% of crude oil by the end of the experiment.

Keywords: PAHs; alkanes; biodegradation; crude oil; field trials; microbial consortia.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Plasmid profile of the studied Rhodococcus degraders. Ladder—λ-phage (BioRad); 1—K3; 2—F2-2; 3—T3-4; 4—K1; 5—T3; 6—K2; 7—B1; and 8—F2-1.
Figure 2
Figure 2
Degradation of oil by different microbial consortia when cultured in Evans liquid mineral medium containing 2% oil for 10 days at 24 °C (minus abiotic degradation); abiotic oil loss was 19 ± 5%. Bars—standard deviation.
Figure 3
Figure 3
Oil removal in the soil in the control plot and the biopreparation-treated plot (bars—standard deviation).
See this image and copyright information in PMC

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