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. 2023 Feb 15;28(4):1839.
doi: 10.3390/molecules28041839.

Standardization of the Optimum Effects of Indole 3-Butyric Acid (IBA) to Control Root Knot Nematode, Meloidogyne enterolobii, in Guava (Psidium guajava L.)

Ashokkumar Natarajan  1 , Dharani Selvam  2 , Kalaiarasan Palaniappan  2 , Akshaya Subbaiah Balamurali  1 , Chandrasekaran Perumal  1 , Rameshkumar Durai  1 , Shakila Sadasivam  1 , Akino Asokan  1 , Ramadass Sivalingam  1 , Ashok Subiramaniyan  3
Affiliations

Standardization of the Optimum Effects of Indole 3-Butyric Acid (IBA) to Control Root Knot Nematode, Meloidogyne enterolobii, in Guava (Psidium guajava L.)

Ashokkumar Natarajan et al. Molecules. .

Abstract

Guava is an important revenue generating crop for small, medium, and commercial guava cultivators all over the world. Nematode infestation is one of the factors that cause declines in fruit production. Researches have proven that the application of plant growth regulators induces the synthesis of defense-related proteins in Guava. IBA is one such plant growth regulator, and its effects on guava plants has not yet been elucidated. Thus, this research is focused on the optimization of IBA concentrations, which results in the induction and production of maximum defense-related proteins to defend against root knot nematode. The present study includes the application of IBA on M. enterolobii-infested experimental guava plants at different concentrations ranging from 100 ppm to 2000 ppm. The synthesis of defense-related proteins is identified with 1000 ppm of IBA. At this concentration, IBA influences the morphological, physiological, and biochemical characteristics and enhances the induction of defense-related proteins in M. enterolobii-infested experimental guava plants. Thus, 1000 ppm of IBA prevents nematode infestation in Lucknow-49 guava plants.

Keywords: M. enterolobii; biochemical; guava; indole 3-butyric acid; morphological; physiological.

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Conflict of interest statement

All authors declared that there is no conflict of interest.

Figures

Figure 1
Figure 1
Effects of indole 3-butyric acid (IBA) on root knot nematode, M. enterolobii, in guava plants.
Figure 2
Figure 2
Influence of different plant growth regulators in guava infected by M. enterolobii at 45, 75, and 105 days after planting: (A) plant height; (B) root length; (C) fresh root biomass; (D) dry root biomass; (E) chlorophyll index; (F) photosynthetic rate; (G) transpiration rate; (H) stomatal conductance; (I) chlorophyll fluorescence; (J) peroxidase; (K) polyphenol oxidase; (L) phenylalanine ammonia lyase; (M) total phenols; (N) acid phosphatase.
Figure 2
Figure 2
Influence of different plant growth regulators in guava infected by M. enterolobii at 45, 75, and 105 days after planting: (A) plant height; (B) root length; (C) fresh root biomass; (D) dry root biomass; (E) chlorophyll index; (F) photosynthetic rate; (G) transpiration rate; (H) stomatal conductance; (I) chlorophyll fluorescence; (J) peroxidase; (K) polyphenol oxidase; (L) phenylalanine ammonia lyase; (M) total phenols; (N) acid phosphatase.
See this image and copyright information in PMC

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