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. 2023:2646:71-82.
doi: 10.1007/978-1-0716-3060-0_7.

Site-Directed Cross-Linking Between Bacterial Flagellar Motor Proteins In Vivo

Hiroyuki Terashima  1 , Michio Homma  2 , Seiji Kojima  2
Affiliations

Site-Directed Cross-Linking Between Bacterial Flagellar Motor Proteins In Vivo

Hiroyuki Terashima et al. Methods Mol Biol. 2023.

Abstract

The bacterial flagellum employs a rotary motor embedded on the cell surface. The motor consists of the stator and rotor elements and is driven by ion influx (typically H+ or Na+) through an ion channel of the stator. Ion influx induces conformational changes in the stator, followed by changes in the interactions between the stator and rotor. The driving force to rotate the flagellum is thought to be generated by changing the stator-rotor interactions. In this chapter, we describe two methods for investigating the interactions between the stator and rotor: site-directed in vivo photo-crosslinking and site-directed in vivo cysteine disulfide crosslinking.

Keywords: Bacterial flagellum; Disulfide cross-link; Flagellar motor; FliG; MotA; Photo-cross-link; PomA; Rotary motor; Rotor; Stator.

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