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. 2023:2646:373-381.
doi: 10.1007/978-1-0716-3060-0_31.

Swimming Motility Assays of Spiroplasma

Daisuke Nakane  1
Affiliations

Swimming Motility Assays of Spiroplasma

Daisuke Nakane. Methods Mol Biol. 2023.

Abstract

Spiroplasma swim in liquids without the use of the bacterial flagella. This small helical bacterium propels itself by generating kinks that travel down the cell body. The kink translation is unidirectional, from the leading pole to the lagging pole, during cell swimming in viscous environments. This protocol describes a swimming motility assay of Spiroplasma eriocheiris for visualizing kink translations of the absolute handedness of the body helix with optical microscopy.

Keywords: Cell motility; Cell polarity; Helical shape; Mollicutes; Optical microscopy.

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References

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