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. 2023 Feb 13;8(7):7119-7127.
doi: 10.1021/acsomega.2c08102. eCollection 2023 Feb 21.

Functional Studies of Cytogenotoxic Potential of Laticifer Proteins of Calotropis procera against Viral Disease

Uzma Saher  1   2 , Muhammad Ovais Omer  1 , Aqeel Javeed  1 , Aftab Ahmad Anjum  3 , Kanwal Rehman  2 , Tanzeela Awan  2 , Gulbeena Saleem  4 , Ameena Mobeen  5
Affiliations

Functional Studies of Cytogenotoxic Potential of Laticifer Proteins of Calotropis procera against Viral Disease

Uzma Saher et al. ACS Omega. .

Abstract

Plant products are widely used for health and disease management. However, besides their therapeutic effects, some plants also have potential toxic activity. Calotropis procera is a well-known laticifer plant having pharmacologically active proteins playing a therapeutically significant role in curing diseases like inflammatory disorders, respiratory diseases, infectious diseases, and cancers. The present study was aimed to investigate the antiviral activity and toxicity profile of the soluble laticifer proteins (SLPs) obtained from C. procera. Different doses of rubber free latex (RFL) and soluble laticifer protein (ranging from 0.019 to 10 mg/mL) were tested. RFL and SLPs were found to be active in a dose-dependent manner against NDV (Newcastle disease virus) in chicken embryos. Embryotoxicity, cytotoxicity, genotoxicity, and mutagenicity of RFL and SLP were examined on chicken embryos, BHK-21 cell lines, human lymphocytes, and Salmonella typhimurium, respectively. It was revealed that RFL and SLP possess embryotoxic, cytotoxic, genotoxic, and mutagenic activity at higher doses (i.e., 1.25-10 mg/mL), while low doses were found to be safe. It was also observed that SLP showed a rather safer profile as compared to RFL. This might be due to the filtration of some small molecular weight compounds at the time of purification of SLPs through a dialyzing membrane. We suggest that SLPs could be used therapeutically against viral disorders but the dose should be critically monitored.

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Conflict of interest statement

The authors declare no competing financial interest.

Figures

Figure 1
Figure 1
SDS–PAGE analysis of C. procera laticifer proteins (RFL and SLP).
Figure 2
Figure 2
Antiviral activity of RFL and SLP obtained from C. procera.
Figure 3
Figure 3
Cell survival percentage (CSP) of C. procera laticifer proteins on BHK-21 cell line.
Figure 4
Figure 4
Genotoxicity evaluation by comet assay for C. procera laticifer proteins.
Figure 5
Figure 5
Revertant colonies of Salmonella typhimurium TA98 with C. procera laticifer proteins (RFL and SLP).
See this image and copyright information in PMC

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