Comparison of Serological Methods for Tick-Borne Encephalitis Virus-Specific Antibody Detection in Wild Boar and Sheep: Impact of the Screening Approach on the Estimated Seroprevalence

Abstract

Tick-borne encephalitis virus (TBEV) is a flavivirus transmitted by ticks. Serological screenings in animals are performed to estimate the prevalence and distribution of TBEV. Most screenings consist of a primary screening by ELISA, followed by confirmation of positive samples by plaque reduction neutralization tests (PRNTs). In this study, 406 wild boar sera were tested with 2 regularly used commercial ELISAs for flavivirus screening in animals (Immunozym FSME (TBEV) IgG All Species (Progen) and ID Screen West Nile Competition (Innovative Diagnostics)) and PRNTs for TBEV and USUTU virus. The results showed that the Immunozym and IDScreen ELISAs had low relative sensitivities of 23% and 20%, respectively, compared to the PRNT results. The relative specificities were 88% and 84% due to cross reactions with USUTU virus-specific antibodies. The minimal TBEV prevalence in our sample set was 8.6% when determined by PRNT. When the screening approach of ELISA testing followed by PRNT confirmation was applied, a TBEV seroprevalence of only 2.0% and 1.7% was found. The suboptimal performance of the ELISAs was confirmed by testing sera collected from experimentally TBEV-infected sheep. While the PRNT detected TBEV specific antibodies in 94% of samples collected between 7 and 18 days post-infection, the ELISAs classified only 50% and 31% of the samples as positive. Both routinely used ELISAs for TBEV antibody screening in animal sera were shown to have a low sensitivity, potentially leading to an underestimation of the true prevalence, and furthermore cross-react with other flavivirus antibodies.

Keywords: ELISA; diagnostic; seroprevalence; tick-borne encephalitis virus.

Figure 1

PRNT titers obtained in sera (n = 139) from commercial pigs without outdoor access.

Figure 2

Relative diagnostic performance of the Immunozym ELISA compared to PRNT for detection of anti-TBEV-specific antibodies in wild boar sera. (a) Comparative table of the results obtained in ELISA to the golden standard. (b) Distribution of the ELISA results in function of the PRNT status and titer.

Figure 3

Relative diagnostic performance of the IDScreen ELISA compared to PRNT for detection of anti-TBEV-specific antibodies in wild boar sera. (a) Comparative table of the results obtained by ELISA to the golden standard. (b) Distribution of the ELISA results in function of the PRNT status and titer.

Figure 4

Quantitative ELISA results versus PRNT titers obtained in sera collected between 1 and 18 days post-experimental TBEV infection of sheep. Results were obtained with the Immunozym ELISA (a) and the IDScreen ELISA (b).