Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1987;26(4):295-300.
doi: 10.1007/BF01221975.

Ultraviolet inactivation and photoreactivation of the cholera phage 'kappa'

S A Samad  1 , S C Bhattacharyya, S N Chatterjee
Affiliations

Ultraviolet inactivation and photoreactivation of the cholera phage 'kappa'

S A Samad et al. Radiat Environ Biophys. 1987.

Abstract

The lysogenic cholera phage, 'Kappa' is some ten to twenty folds more resistant to UV (254 nm) than are most of the T. phages of E. coli, or the cholera phage PL 163/10, or the host V. cholerae strain H218 Smr, the 37% (D37) and 10% (D10) survival doses being 255.8 J/m2 and 633.6 J/m2 respectively. The UV-irradiated 'Kappa' phages could be photoreactivated in the host V. cholerae strain H218 Smr to a maximum extent of 40%. The removal of the number of lethal hits per phage by the survival-enhancement treatment (photoreactivation) with time followed an exponential relation, the constant probability of removal of lethal hit per unit time being 2.8 x 10(-2)min-1. The UV-irradiated phages could also be Weigle reactivated in the host strain H218 Smr by a small degree, the maximum reactivation factor (ratio of survivals in UV-irradiated and non-irradiated hosts) being 1.50.

PubMed Disclaimer

References

    1. Photochem Photobiol. 1986 Apr;43(4):413-21 - PubMed
    1. Virology. 1964 Nov;24:349-58 - PubMed
    1. Genetics. 1949 Mar;34(2):93-125 - PubMed
    1. Can J Microbiol. 1976 Aug;22(8):1186-7 - PubMed
    1. Biochem Biophys Res Commun. 1976 Jul 12;71(1):91-8 - PubMed

MeSH terms