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. 2023 Mar 27:7:287.
doi: 10.12688/wellcomeopenres.18445.2. eCollection 2022.

The genome sequence of the malaria mosquito, Anopheles funestus, Giles, 1900

Diego Ayala  1   2 , Ousman Akone-Ella  2 , Pierre Kengne  1   2 , Harriet Johnson  3 , Haynes Heaton  4 , Joanna Collins  5 , Ksenia Krasheninnikova  5 , Sarah Pelan  5 , Damon-Lee Pointon  5 , Ying Sims  5 , James Torrance  5 , Alan Tracey  5 , Marcela Uliano-Silva  5 , Katharina von Wyschetzki  5 , Jonathan Wood  5 ; DNA Pipelines collective; Shane McCarthy  5   6 , Daniel Neafsey  7   8 , Alex Makunin  5 , Mara Lawniczak  5
Affiliations

The genome sequence of the malaria mosquito, Anopheles funestus, Giles, 1900

Diego Ayala et al. Wellcome Open Res. .

Abstract

We present a genome assembly from an individual female Anopheles funestus (the malaria mosquito; Arthropoda; Insecta; Diptera; Culicidae). The genome sequence is 251 megabases in span. The majority of the assembly is scaffolded into three chromosomal pseudomolecules with the X sex chromosome assembled. The complete mitochondrial genome was also assembled and is 15.4 kilobases in length.

Keywords: African malaria mosquito; Anopheles funestus; chromosomal inversions; genome sequence.

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Conflict of interest statement

No competing interests were disclosed.

Figures

Figure 1.
Figure 1.. Genome assembly of Anopheles funestus, idAnoFuneDA-416_04: metrics.
The BlobToolKit Snailplot shows N50 metrics and BUSCO gene completeness. The main plot is divided into 1,000 size-ordered bins around the circumference with each bin representing 0.1% of the 250,713,484 bp assembly. The distribution of chromosome lengths is shown in dark grey with the plot radius scaled to the longest chromosome present in the assembly (102,883,511 bp, shown in red). Orange and pale-orange arcs show the N50 and N90 chromosome lengths (84,636,641 and 355,752 bp), respectively. The pale grey spiral shows the cumulative chromosome count on a log scale with white scale lines showing successive orders of magnitude. The blue and pale-blue area around the outside of the plot shows the distribution of GC, AT and N percentages in the same bins as the inner plot. A summary of complete, fragmented, duplicated and missing BUSCO genes in the diptera_odb10 set is shown in the top right. An interactive version of this figure is available at https://blobtoolkit.genomehubs.org/view/Anopheles funestus/dataset/CALSEJ01/snail.
Figure 2.
Figure 2.. Genome assembly of Anopheles funestus, idAnoFuneDA-416_04: GC coverage.
BlobToolKit GC-coverage plot. An interactive version of this figure is available at https://blobtoolkit.genomehubs.org/view/Anopheles funestus/dataset/CALSEJ01/blob#Filters.
Figure 3.
Figure 3.. Genome assembly of Anopheles funestus, idAnoFuneDA-416_04: cumulative sequence.
BlobToolKit cumulative sequence plot. An interactive version of this figure is available at https://blobtoolkit.genomehubs.org/view/Anopheles funestus/dataset/CALSEJ01/cumulative#Filters.
Figure 4.
Figure 4.. Genome assembly of Anopheles funestus, idAnoFuneDA-416_04: Hi-C contact map.
Hi-C contact map of the idAnoFuneDA- 416_04 assembly, visualised in HiGlass. Chromosomes are arranged in size order from left to right and top to bottom. The interactive Hi-C map can be viewed at https://genome-note-higlass.tol.sanger.ac.uk/l/?d=aJmC2VieTlCIjrBC-4LXsA.
Figure 5.
Figure 5.. Synteny between genome assemblies of Anopheles funestus, AfunF3 and idAnoFuneDA-416_04.
Black dots represent locations of assembly gaps, blue vertical lines – approximate centromere locations. Four largest inversions identified as 2Rh, 3Ra, 3Rb, and 3La ( Table 3).
See this image and copyright information in PMC

References

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