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. 2023 Mar 6;23(1):71.
doi: 10.1186/s12906-023-03854-8.

Senna occidentalis (L.) Link root extract inhibits Plasmodium growth in vitro and in mice

Simeon Mogaka  1   2 , Halkano Molu  3 , Esther Kagasi  3 , Kenneth Ogila  4 , Rebeccah Waihenya  4 , Faith Onditi  3   5 , Hastings Ozwara  3
Affiliations

Senna occidentalis (L.) Link root extract inhibits Plasmodium growth in vitro and in mice

Simeon Mogaka et al. BMC Complement Med Ther. .

Abstract

Background: Senna occidentalis (L.) Link has been used worldwide in traditional treatment of many diseases and conditions including snakebite. In Kenya, a decoction from the plant roots taken orally, is used as a cure for malaria. Several studies have demonstrated that extracts from the plant possess antiplasmodial activity, in vitro. However, the safety and curative potency of the plant root against established malaria infection is yet to be scientifically validated, in vivo. On the other hand, there are reports on variation in bioactivity of extracts obtained from this plant species, depending on the plant part used and place of origin among other factors. In this study, we demonstrated the antiplasmodial activity of Senna occidentalis roots extract in vitro, and in mice.

Methods: Methanol, ethyl acetate, chloroform, hexane and water extracts of S. occidentalis root were tested for in vitro antiplasmodial activity against Plasmodium falciparum, strain 3D7. Cytotoxicity of the most active solvent extracts was determined using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and the curative potency in Plasmodium berghei infected mice evaluated by Rane's test.

Results: All of the solvent extracts tested in this study inhibited the propagation of P. falciparum, strain 3D7, in vitro, with polar extracts being more active than non-polar ones. Methanolic extracts had the highest activity (IC50 = 1.76) while hexane extract displayed the lowest activity (IC50 = 18.47). At the tested concentrations, methanolic and aqueous extracts exhibited high selectivity index against P. falciparum strain 3D7 (SI > 10) in the cytotoxicity assay. Further, the extracts significantly suppressed the propagation of P. berghei parasites (P < 0.05) in vivo and increased the survival time of the infected mice (P < 0.0001).

Conclusions: Senna occidentalis (L.) Link root extract inhibits the propagation of malaria parasites in vitro and in BALB/c mice.

Keywords: Ethnomedicine; In vitro; In vivo; Malaria; Plasmodium; Senna occidentalis.

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Conflict of interest statement

There are no competing interests to disclose.

Figures

Fig. 1
Fig. 1
Parasitemic profile of pyrimethamine-sensitive Plasmodium berghei 7 days upon extract treatment, in comparison with controls
Fig. 2
Fig. 2
Survivorship curves of Plasmodium berghei infected upon treatment with the extracts
See this image and copyright information in PMC

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References

    1. Baah MK, Mensah AY, Asante-Kwatia E, Amponsah IK, Forkuo AD, Harley BK, et al. In vivo antiplasmodial activity of different solvent extracts of Myrianthus libericus stem bark and its constituents in plasmodium berghei-infected mice. Evid Based Complement Alternat Med. 2020;2020. ID: 8703197. - PMC - PubMed
    1. Cimanga RK, Nsaka SL, Tshodi ME, Mbamu BM, Kikweta CM, Makila FB-M, et al. In vitro and in vivo antiplasmodial activity of extracts and isolated constituents of Alstonia congensis root bark. J Ethnopharmacol. 2019;242:111736. doi: 10.1016/j.jep.2019.02.019. - DOI - PubMed
    1. WHO . World malaria report 2022. World Health Organization; 2022.
    1. Chaniad P, Techarang T, Phuwajaroanpong A, Punsawad C. Antimalarial activity and toxicological assessment of Betula alnoides extract against plasmodium berghei infections in mice. Evid Based Complement Alternat Med. 2019;2019. ID: 2324679. - PMC - PubMed
    1. Ekasari W, Wahyuni TS, Arwaty H, Putri NT. Determination of effective dose of antimalarial from Cassia spectabilis leaf ethanol extract in plasmodium berghei-infected mice. Afr J Infect Diseases. 2018;12(1S):110–115. - PMC - PubMed

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