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. 2023:2636:263-277.
doi: 10.1007/978-1-0716-3012-9_15.

Rapid Testing of Gene Function in Axonal Regeneration After Spinal Cord Injury Using Larval Zebrafish

Louisa K Drake  1 , Marcus Keatinge  1 , Themistoklis M Tsarouchas  1 , Catherina G Becker  1   2 , David A Lyons  1 , Thomas Becker  3   4
Affiliations

Rapid Testing of Gene Function in Axonal Regeneration After Spinal Cord Injury Using Larval Zebrafish

Louisa K Drake et al. Methods Mol Biol. 2023.

Abstract

Larval zebrafish show axonal regrowth over a complex spinal injury site and recovery of function within days after injury. Here we describe a simple protocol to disrupt gene function in this model using acute injections of highly active synthetic gRNAs to rapidly detect loss-of-function phenotypes without the need for breeding.

Keywords: Axon regeneration; CRISPR/Cas9 mutagenesis; Spinal cord injury; Zebrafish larvae.

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References

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