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. 2023 Aug;252(8):1113-1129.
doi: 10.1002/dvdy.582. Epub 2023 Mar 16.

Microglial morphology in the somatosensory cortex across lifespan. A quantitative study

Sanziana Godeanu  1   2 , Devin Clarke  3 , Laura Stopper  2 , Alexandru-Florian Deftu  4   5 , Aurel Popa-Wagner  1 , Adrian Tudor Bălșeanu  1 , Anja Scheller  2 , Bogdan Catalin  1   2
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Free article

Microglial morphology in the somatosensory cortex across lifespan. A quantitative study

Sanziana Godeanu et al. Dev Dyn. 2023 Aug.
Free article

Abstract

Background: Microglia are long-lived cells that constantly monitor their microenvironment. To accomplish this task, they constantly change their morphology both in the short and long term under physiological conditions. This makes the process of quantifying physiological microglial morphology difficult.

Results: By using a semi-manual and a semi-automatic method to assess fine changes in cortical microglia morphology, we were able to quantify microglia changes in number, surveillance and branch tree starting from the fifth postnatal day to 2 years of life. We were able to identify a fluctuating behavior of most analyzed parameters characterized by a rapid cellular maturation, followed by a long period of relative stable morphology during the adult life with a final convergence to an aged phenotype. Detailed cellular arborization analysis revealed age-induced differences in microglia morphology, with mean branch length and the number of terminal processes changing constantly over time.

Conclusions: Our study provides insight into microglia morphology changes across lifespan under physiological conditions. We were able to highlight, that due to the dynamic nature of microglia several morphological parameters are needed to establish the physiological state of these cells.

Keywords: cortex; lifespan changes; microglia arborization.

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References

REFERENCES

    1. Davalos D, Grutzendler J, Yang G, et al. ATP mediates rapid microglial response to local brain injury in vivo. Nat Neurosci. 2005;8(6):752-758. doi:10.1038/nn1472
    1. Nimmerjahn A, Kirchhoff F, Helmchen F. Resting microglial cells are highly dynamic surveillants of brain parenchyma in vivo. Science. 2005;308(5726):1314-1318. doi:10.1126/science.1110647
    1. Catalin B, Cupido A, Iancau M, Albu CV, Kirchhoff F. Microglia: first responders in the central nervous system. Rom J Morphol Embryol. 2013;54(3):467-472.
    1. Weinhard L, di Bartolomei G, Bolasco G, et al. Microglia remodel synapses by presynaptic trogocytosis and spine head filopodia induction. Nat Commun. 2018;9(1):1228. doi:10.1038/s41467-018-03566-5
    1. Catalin B, Stopper L, Balseanu T-A, Scheller A. The in situ morphology of microglia is highly sensitive to the mode of tissue fixation. J Chem Neuroanat. 2017;86:59-66. doi:10.1016/j.jchemneu.2017.08.007

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