Severely ill COVID-19 patients have altered circulating levels of proteins controlling the epitranscriptome

A Quiles-Jiménez¹, M M L Sousa², C Huse³, A M Dyrhol-Riise⁴, J C Holter⁵, E E Christensen⁴, K Tonby⁴, A R Holten⁶, P Aukrust⁷, M Bjørås⁸, T B Dahl⁹, B Halvorsen¹⁰

Affiliations

¹ Research Institute of Internal Medicine, Oslo University Hospital, Oslo, Norway; Institute of Clinical Medicine, University of Oslo, Oslo, Norway. Electronic address: amquiles@uio.no.
² Department of Clinical and Molecular Medicine, Norwegian University of Science and Technology, NTNU, Trondheim, Norway; PROMEC Core Facility for Proteomics and Metabolomics, NTNU and the Central Norway Regional Health Authority, Trondheim, Norway.
³ Research Institute of Internal Medicine, Oslo University Hospital, Oslo, Norway; Institute of Clinical Medicine, University of Oslo, Oslo, Norway.
⁴ Institute of Clinical Medicine, University of Oslo, Oslo, Norway; Department of Infectious Diseases, Oslo University Hospital, Oslo, Norway.
⁵ Institute of Clinical Medicine, University of Oslo, Oslo, Norway; Department of Microbiology, Oslo University Hospital, Oslo, Norway.
⁶ Institute of Clinical Medicine, University of Oslo, Oslo, Norway; Department of Acute Medicine, Oslo University Hospital, Oslo, Norway.
⁷ Research Institute of Internal Medicine, Oslo University Hospital, Oslo, Norway; Institute of Clinical Medicine, University of Oslo, Oslo, Norway; Section of Clinical Immunology and Infectious Diseases, Oslo University Hospital, Oslo, Norway.
⁸ Department of Clinical and Molecular Medicine, Norwegian University of Science and Technology, NTNU, Trondheim, Norway; Department of Microbiology, Oslo University Hospital, Oslo, Norway.
⁹ Research Institute of Internal Medicine, Oslo University Hospital, Oslo, Norway.
¹⁰ Research Institute of Internal Medicine, Oslo University Hospital, Oslo, Norway; Institute of Clinical Medicine, University of Oslo, Oslo, Norway; Department of Medicine, Division of Cardiovascular Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA.

PMID: 36889510
PMCID: PMC9987596
DOI: 10.1016/j.jinf.2023.03.002

Comment

Severely ill COVID-19 patients have altered circulating levels of proteins controlling the epitranscriptome

A Quiles-Jiménez et al. J Infect. 2023 Jun.

. 2023 Jun;86(6):593-595.

doi: 10.1016/j.jinf.2023.03.002. Epub 2023 Mar 6.

Authors

A Quiles-Jiménez¹, M M L Sousa², C Huse³, A M Dyrhol-Riise⁴, J C Holter⁵, E E Christensen⁴, K Tonby⁴, A R Holten⁶, P Aukrust⁷, M Bjørås⁸, T B Dahl⁹, B Halvorsen¹⁰

Affiliations

¹ Research Institute of Internal Medicine, Oslo University Hospital, Oslo, Norway; Institute of Clinical Medicine, University of Oslo, Oslo, Norway. Electronic address: amquiles@uio.no.
² Department of Clinical and Molecular Medicine, Norwegian University of Science and Technology, NTNU, Trondheim, Norway; PROMEC Core Facility for Proteomics and Metabolomics, NTNU and the Central Norway Regional Health Authority, Trondheim, Norway.
³ Research Institute of Internal Medicine, Oslo University Hospital, Oslo, Norway; Institute of Clinical Medicine, University of Oslo, Oslo, Norway.
⁴ Institute of Clinical Medicine, University of Oslo, Oslo, Norway; Department of Infectious Diseases, Oslo University Hospital, Oslo, Norway.
⁵ Institute of Clinical Medicine, University of Oslo, Oslo, Norway; Department of Microbiology, Oslo University Hospital, Oslo, Norway.
⁶ Institute of Clinical Medicine, University of Oslo, Oslo, Norway; Department of Acute Medicine, Oslo University Hospital, Oslo, Norway.
⁷ Research Institute of Internal Medicine, Oslo University Hospital, Oslo, Norway; Institute of Clinical Medicine, University of Oslo, Oslo, Norway; Section of Clinical Immunology and Infectious Diseases, Oslo University Hospital, Oslo, Norway.
⁸ Department of Clinical and Molecular Medicine, Norwegian University of Science and Technology, NTNU, Trondheim, Norway; Department of Microbiology, Oslo University Hospital, Oslo, Norway.
⁹ Research Institute of Internal Medicine, Oslo University Hospital, Oslo, Norway.
¹⁰ Research Institute of Internal Medicine, Oslo University Hospital, Oslo, Norway; Institute of Clinical Medicine, University of Oslo, Oslo, Norway; Department of Medicine, Division of Cardiovascular Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts, USA.

PMID: 36889510
PMCID: PMC9987596
DOI: 10.1016/j.jinf.2023.03.002

No abstract available

PubMed Disclaimer

Conflict of interest statement

Declaration of Competing Interest Nothing to declare.

Figures

**Fig. 1**
Relative protein levels of the m⁶A- (A) writers WTAP, METTL3, METTL14, METTL5 and DIMT1, (B) erasers FTO and ALKBH5, and (C) readers YTHDF2, YTHDF3 and eIF3A. Data is relative to GAPDH and presented as box and whiskers showing the minimum and maximum values of PBMC samples from healthy controls (HC; n = 11), non-respiratory failure COVID-19 patients (Non-RF; n = 12), and respiratory failure COVID-19 patients (RF; n = 6). Unpaired t-test performed for statistical significance: ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. Panel (D) shows Pearson’s correlation analyses with 95% confidence interval of statistically significant (p < 0.05) findings between protein levels and P/F mean in COVID-19 patient samples.

See this image and copyright information in PMC

Comment in

Genetic variant in complement receptor 1 (CR1, CD35) is associated with a cluster of severe fatal COVID-19 in a family.
Danial-Farran N, Khlaila M, Avraham-Kelbert M, Chazan B, Khayat M, Winder A, Shalev S, Bisharat N. Danial-Farran N, et al. J Infect. 2023 Oct;87(4):336-338. doi: 10.1016/j.jinf.2023.03.014. Epub 2023 Mar 15. J Infect. 2023. PMID: 36924837 Free PMC article. No abstract available.

Comment on

Serum IFN-γ and RNAemia temporal profiles as biomarkers of severe COVID-19 in solid organ transplant and immunocompetent patients.
Salto-Alejandre S, Carretero-Ledesma M, Camacho-Martínez P, Berastegui-Cabrera J, Infante C, Rodríguez-Álvarez R, Alba J, Pérez-Palacios P, García-Díaz E, Roca C, Praena J, Blanco-Vidal MJ, Santibáñez S, Valverde-Ortiz R, Nieto-Arana J, García-García C, Gutiérrez-Campos D, Maldonado N, Bernal G, Gómez-Bravo MÁ, Sobrino JM, Aguilar-Guisado M, Álvarez-Marín R, Goikoetxea-Aguirre J, Oteo JA, Palacios-Baena ZR, Pascual Á, Lepe JA, Rodríguez-Baño J, Cisneros JM, Pachón J, Sánchez-Céspedes J, Cordero E; COVIDSOT Working Team. Salto-Alejandre S, et al. J Infect. 2023 May;86(5):529-533. doi: 10.1016/j.jinf.2023.01.019. Epub 2023 Jan 21. J Infect. 2023. PMID: 36690212 Free PMC article. No abstract available.

References

1. Salto-Alejandre S., Carretero-Ledesma M., Camacho-Martínez P., Berastegui-Cabrera J., Infante C., Rodríguez-Álvarez R., et al. Serum IFN-gamma and RNAemia temporal profiles as biomarkers of severe COVID-19 in solid organ transplant and immunocompetent patients. J Infect. 2023;0 doi: 10.1016/j.jinf.2023.01.019. S0163-4453(23)00024-5. - DOI - PMC - PubMed
1. Christensen E.E., Jørgensen M.J., Nore K.G., Dahl T.B., Yang K., Ranheim T., et al. Critical COVID-19 is associated with distinct leukocyte phenotypes and transcriptome patterns. J Intern Med. 2021;290:677–692. doi: 10.1111/joim.13310. - DOI - PMC - PubMed
1. Phillips S., Mishra T., Khadka S., Bohan D., Espada C.E., Maury W., et al. Epitranscriptomic N6-methyladenosine profile of SARS-CoV-2-infected human lung epithelial cells. Microbiol Spectr. 2023;0 doi: 10.1128/spectrum.03943–22. e03943-03922. - DOI - PMC - PubMed
1. Qiu X., Hua X., Li Q., Zhou Q., Chen J. m6A regulator-mediated methylation modification patterns and characteristics of immunity in blood leukocytes of COVID-19 patients. Front Immunol. 2021;12 doi: 10.3389/fimmu.2021.774776. - DOI - PMC - PubMed
1. Qing X., Chen Q., Wang K. m6A regulator-mediated methylation modification patterns and characteristics in COVID-19 patients. Front Public Health. 2022;10 doi: 10.3389/fpubh.2022.914193. - DOI - PMC - PubMed

Publication types

Actions
Actions
Actions

MeSH terms

Actions
Actions
Actions

LinkOut - more resources

Full Text Sources
Medical
- MedlinePlus Health Information

Save citation to file

Email citation

Add to Collections

Add to My Bibliography

Your saved search

Create a file for external citation management software

Your RSS Feed

Severely ill COVID-19 patients have altered circulating levels of proteins controlling the epitranscriptome

Affiliations

Severely ill COVID-19 patients have altered circulating levels of proteins controlling the epitranscriptome

Authors

Affiliations

Conflict of interest statement

Figures

Comment in

Comment on

References

Publication types

MeSH terms

LinkOut - more resources

Full Text Sources

Medical