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. 2023:2651:69-83.
doi: 10.1007/978-1-0716-3084-6_5.

NMR Titration Studies in Z-DNA Dynamics

Seo-Ree Choi  1 , Kwang-Im Oh  1 , Yeo-Jin Seo  1 , Joon-Hwa Lee  2
Affiliations

NMR Titration Studies in Z-DNA Dynamics

Seo-Ree Choi et al. Methods Mol Biol. 2023.

Abstract

Chemical shift perturbation (CSP) is a simple NMR technique for studying the DNA binding of proteins. Titration of the unlabeled DNA into the 15N-labeled protein is monitored by acquiring a two-dimensional (2D) heteronuclear single-quantum correlation (HSQC) spectrum at each step of the titration. CSP can also provide information on the DNA-binding dynamics of proteins, as well as protein-induced conformational changes in DNA. Here, we describe the titration of DNA for the 15N-labeled Z-DNA-binding protein, monitored via 2D HSQC spectra. NMR titration data can be analyzed with the active B-Z transition model to provide the protein-induced B-Z transition dynamics of DNA.

Keywords: Chemical shift perturbation; DNA–protein interaction; Dynamics; NMR; Titration; Z-DNA; Z-DNA-binding protein.

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