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. 2023 Feb 21;28(5):2032.
doi: 10.3390/molecules28052032.

Enhancing the Antimicrobial Effect of Ozone with Mentha piperita Essential Oil

Alin-Daniel Floare  1   2 , Ramona Dumitrescu  1   2 , Vlad Tiberiu Alexa  1   2 , Octavia Balean  1   2 , Camelia Szuhanek  2   3 , Diana Obistioiu  4 , Ileana Cocan  5 , Alina-Georgeta Neacsu  6 , Iuliana Popescu  6 , Aurora Doris Fratila  7 , Atena Galuscan  1   2
Affiliations

Enhancing the Antimicrobial Effect of Ozone with Mentha piperita Essential Oil

Alin-Daniel Floare et al. Molecules. .

Abstract

This study aimed to obtain and analyse Mentha piperita essential oil (MpEO) for the prospect of being used as an enhancement agent for the antimicrobial potential of ozone against gram-positive and gram-negative bacteria and fungi. The research was done for different exposure times, and it gained time-dose relationships and time-effect correlations. Mentha piperita (Mp) essential oil (MpEO) was obtained via hydrodistillation and further analysed by using GC-MS. The broth microdilution assay was used to determine the strain inhibition/strain mass growth by using spectrophotometric optical density reading (OD). The bacterial/mycelium growth rates (BGR/MGR) and the bacterial/mycelium inhibition rates (BIR/MIR) after ozone treatment in the presence and absence of MpEO on the ATTC strains were calculated; the minimum inhibition concentration (MIC) and statistical interpretations of the time-dose relationship and specific t-test correlations were determined. The effect of ozone on the following tested strains at maximum efficiency was observed after 55 s of single ozone exposure, in order of effect strength: S. aureus > P. aeruginosa > E. coli > C. albicans > S. mutans. For ozone with the addition of 2% MpEO (MIC), maximum efficacy was recorded at 5 s for these strains, in order of effect strength: C. albicans > E. coli > P. aeruginosa > S. aureus > S. mutans. The results suggest a new development and affinity regarding the cell membrane of the different microorganisms tested. In conclusion, the use of ozone, combined with MpEO, is sustained as an alternative therapy in plaque biofilm and suggested as helpful in controlling oral disease-causing microorganisms in medicine.

Keywords: GC-MS; Mentha piperita; essential oil; gram-negative bacteria; gram-positive; ozonation.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
MpEO antimicrobial activity on the tested strains at different concentrations expressed as BIR%.
Figure 2
Figure 2
Ozone and ozone+MpEO antimicrobial activity on S. mutans at different time exposures expressed in seconds (″). (a) Results expressed as BIR%; (b) results expressed as BGR%.
Figure 3
Figure 3
Ozone and ozone+MpEO antimicrobial activity on S. aureus at different time exposures expressed in seconds (″). (a) Results expressed as BIR%; (b) results expressed as BGR%.
Figure 4
Figure 4
Ozone and ozone+MpEO antimicrobial activity on P. aeruginosa at different time exposures expressed in seconds (″). (a) Results expressed as BIR%; (b) results expressed as BGR%.
Figure 5
Figure 5
Ozone and ozone+MpEO antimicrobial activity on E. coli at different time exposures expressed in seconds (″). (a) Results expressed as BIR%; (b) results expressed as BGR%.
Figure 6
Figure 6
Ozone and ozone+MpEO antimicrobial activity tested on C. albicans at different time exposures expressed in seconds (″). (a) Results expressed as MIR%; (b) results expressed as BGR%.
Figure 7
Figure 7
Antimicrobial activity (expressed as BIR/MIR%) of ozone tested on ATCC strains at different exposure times represented in seconds (″).
Figure 8
Figure 8
Antimicrobial activity (expressed as BIR/MIR%) of ozone+MpEO tested on ATCC strains at different exposure times expressed in seconds (″).
See this image and copyright information in PMC

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