Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1987 Dec 24;51(6):987-1000.
doi: 10.1016/0092-8674(87)90585-x.

Expression of a single transfected cDNA converts fibroblasts to myoblasts

R L Davis  1 , H Weintraub, A B Lassar
Affiliations

Expression of a single transfected cDNA converts fibroblasts to myoblasts

R L Davis et al. Cell. .

Abstract

5-azacytidine treatment of mouse C3H10T1/2 embryonic fibroblasts converts them to myoblasts at a frequency suggesting alteration of one or only a few closely linked regulatory loci. Assuming such loci to be differentially expressed as poly(A)+ RNA in proliferating myoblasts, we prepared proliferating myoblast-specific, subtracted cDNA probes to screen a myocyte cDNA library. Based on a number of criteria, three cDNAs were selected and characterized. We show that expression of one of these cDNAs transfected into C3H10T1/2 fibroblasts, where it is not normally expressed, is sufficient to convert them to stable myoblasts. Myogenesis also occurs, but to a lesser extent, when this cDNA is expressed in a number of other cell lines. The major open reading frame encoded by this cDNA contains a short protein segment similar to a sequence present in the myc protein family.

PubMed Disclaimer

Comment in

  • From MYOD1 to iPS cells.
    Hochedlinger K. Hochedlinger K. Nat Rev Mol Cell Biol. 2010 Dec;11(12):817. doi: 10.1038/nrm3018. Nat Rev Mol Cell Biol. 2010. PMID: 21102604 No abstract available.

Publication types

Associated data