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. 2023 Mar 1:31:628-630.
doi: 10.1016/j.omtn.2023.02.012. eCollection 2023 Mar 14.

Innovative microRNA quantification by qPCR

Minh Ngoc Le  1 , Tuan Anh Nguyen  1
Affiliations

Innovative microRNA quantification by qPCR

Minh Ngoc Le et al. Mol Ther Nucleic Acids. .
No abstract available

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Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1
miRNA biogenesis and isoforms (isomiRs) (A) Multiple RNA sequences contain a miRNA sequence. The blue and pink strands represent the 5p and 3p miRNAs, respectively. The green and blue arrowheads indicate the cleavage sites of the Microprocessor and DICER enzymes, respectively. The red arrowheads indicate alternative cleavage sites of Microprocessor and DICER. (B) The human let-7 and miR-30 families. The similar nucleotides shared among all the members in each family are highlighted in red.
Figure 2
Figure 2
Quantification of miRNAs using qPCR methods (A) A comparison of TaqMan qPCR, miRCURY qPCR, and SPLICER-qPCR for miRNA quantification. The abbreviation RT stands for reverse transcription, PCR for polymerase chain reaction, and SMBs for streptavidin magnetic beads. (B) Factors that may impact the specificity of SPLICER-qPCR are shown, including the possibility of cross-reactivity with similar miRNAs in the same family, miRNA precursors, and isomiRs.
See this image and copyright information in PMC

References

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