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. 2023 Mar;3(3):e700.
doi: 10.1002/cpz1.700.

Searching and Navigating UniProt Databases

Yvonne C Lussi  1 , Michele Magrane  1 , Maria J Martin  1 , Sandra Orchard  1 ; UniProt Consortium  1   2   3
Affiliations

Searching and Navigating UniProt Databases

Yvonne C Lussi et al. Curr Protoc. 2023 Mar.

Abstract

The Universal Protein Resource (UniProt) is a comprehensive resource for protein sequence and annotation data. The UniProt website receives about 800,000 unique visitors per month and is the primary means to access UniProt. It provides 10 searchable datasets and four main tools. The key UniProt datasets are the UniProt Knowledgebase (UniProtKB), the UniProt Reference Clusters (UniRef), the UniProt Archive (UniParc), and protein sets for completely sequenced genomes (Proteomes). Other supporting datasets include information about proteins that is present in UniProtKB protein entries, such as literature citations, taxonomy, and subcellular locations, among others. This article focuses on how to use UniProt datasets. The first basic protocol describes navigation and searching mechanisms for the UniProt datasets, and two additional protocols build on the first protocol to describe advanced search and query building. © 2023 The Authors. Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1: Searching UniProt datasets Basic Protocol 2: Advanced search and query building Basis Protocol 3: Adding parameters using advanced search.

Keywords: UniProt; database; navigation; protein; search; tutorial.

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Conflict of interest statement

Conflict of Interest

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
UniProt datasets’ drop-down menu.
Figure 2
Figure 2
UniProtKB search results page.
Figure 3
Figure 3
UniProtKB search results with filter applied.
Figure 4
Figure 4
UniProtKB protein entry INS_HUMAN for human insulin.
Figure 5
Figure 5
UniProtKB advanced search field types available.
Figure 6
Figure 6
Advanced search example.
Figure 7
Figure 7
UniProtKB search results through advanced search.
Figure 8
Figure 8
Adding parameters to UniProtKB advanced search.
Figure 9
Figure 9
UniProtKB search result for additional advanced search parameters.
Figure 10
Figure 10
Evidence tags in INS_HUMAN protein entry.
Figure 11
Figure 11
Highlighted alignment for CENPA_HUMAN sequences.
Figure 12
Figure 12
UniProtKB Bibliography Submission Page.
See this image and copyright information in PMC

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