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. 2023 Sep;396(9):2071-2085.
doi: 10.1007/s00210-023-02446-0. Epub 2023 Mar 14.

Nitidine chloride regulates cell function of bladder cancer in vitro through downregulating Lymphocyte antigen 75

Shi-Shuo Wang  1 , Gao-Qiang Zhai  2 , Zhi-Guang Huang  1 , Jia-Yuan Luo  1 , Juan He  1 , Jie-Zhuang Huang  2 , Ling Yang  2 , Chu-Nan Xiao  2 , Su-Li Li  2 , Kai-Rong Chen  2 , Yan-Yu Chen  2 , Han-Chu Ji  3 , Jun-Ping Ding  4 , Sheng-Hua Li  2 , Ji-Wen Cheng  2 , Gang Chen  5
Affiliations

Nitidine chloride regulates cell function of bladder cancer in vitro through downregulating Lymphocyte antigen 75

Shi-Shuo Wang et al. Naunyn Schmiedebergs Arch Pharmacol. 2023 Sep.

Abstract

Nitidine chloride (NC) is effective on cancer in many tumors, but its effect on bladder cancer (BC) is unknown. We conducted cell function experiments to verify the antineoplastic effect of NC on BC cell lines (5637, T24, and UM-UC-3) in vitro. Then, mRNAs of NC-treated and NC-untreated BC cells were extracted for mRNA sequencing. Differentially expressed genes (DEGs), expression analysis, and drug molecular docking were conducted to discover the target gene of NC. Finally, functional enrichment was analyzed to explore the underlying mechanisms. NC dramatically inhibited proliferation, migration, and invasion, and it induced apoptosis and arrested the S and G2/M phases of BC cell lines. Lymphocyte antigen 75 (LY75) appeared to be the target of NC. LY75 was highly expressed and had the ability to distinguish BC tissue from non-cancerous tissue. Then, drug molecular docking confirmed the targeting relationship between NC and LY75. Gene enrichment analysis showed that the downregulated genes, after being treated with NC, were mainly enriched in pathways relevant to cell pathophysiological processes. NC inhibits BC cell proliferation, migration, and invasion, induces apoptosis, and arrests cell cycles by downregulating the expression of LY75. This study provides molecular and theoretical bases for NC treatment of BC.

Keywords: Bladder cancer; Cell function; LY75; Nitidine chloride; mRNA sequencing.

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