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. 2023 Mar 1;51(2):45-51.
doi: 10.15586/aei.v51i2.789. eCollection 2023.

Effect of transduced mesenchymal stem cells with IL-10 gene on control of allergic asthma

Congxia Hou  1 , Fang Sun  1 , Yalin Liang  2 , Entezar Mehrabi Nasab  3 , Seyyed Shamsadin Athari  4
Affiliations

Effect of transduced mesenchymal stem cells with IL-10 gene on control of allergic asthma

Congxia Hou et al. Allergol Immunopathol (Madr). .

Abstract

Asthma is an important pulmonary disease associated with T helper lymphocyte (Th)2 dominant immune response, which can initiate allergic and inflammatory reactions. Interleukin (IL)-10 is the main immune suppressor cytokine, and mesenchymal stem cells (MSCs) have an immune-modulatory potential that can be transduced with the expression of the IL-10 gene to control pathophysiology of allergic asthma. Bone marrow's MSCs were isolated and transduced with the expression vector that contains the expressible IL-10 gene. Then, allergic asthma mouse model was produced and treated with manipulated MSCs. Methacholine challenge test; measurement of IL-4, IL-5, IL-8, IL-13, IL-25, and IL-33; and total and ovalbumin (OVA)-specific immunoglobulin (Ig)E levels were done. Hyperplasia of the goblet cell, secretion of mucus, and peribronchiolar and perivascular eosinophilic inflammation were evaluated in lung pathological sections. IL-25, IL-33, and total IgE levels; AHR; eosinophilic inflammation; hyperplasia of the goblet cell; and secretion of mucus could be controlled in M, MV, and MV-10 groups, and the control in the MV-10 group was strong compared to M and MV groups. MSCs have immune-modulatory capacity that can control allergic asthma pathophysiology, and this effect can be strengthened and reinforced by the expression of IL-10 gene.

Keywords: Allergy; Cell therapy; Cytokine; Gene therapy; Inflammation.

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Conflict of interest statement

There is no conflict of interest.

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