Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Jan;15(1):31-42.
doi: 10.4155/bio-2022-0230. Epub 2023 Mar 17.

Quantification of levetiracetam in plasma and urine and its application to a pharmacokinetic study of traumatic brain injury patients

Santosh Ks Adiraju  1 , Chandra D Sumi  1 , Saurabh Pandey  1 , Menino O Cotta  1 , Jason A Roberts  1   2   3   4 , Jeffrey Lipman  1   2   3   5 , Steven C Wallis  1 , Suzanne L Parker  1
Affiliations
Free article

Quantification of levetiracetam in plasma and urine and its application to a pharmacokinetic study of traumatic brain injury patients

Santosh Ks Adiraju et al. Bioanalysis. 2023 Jan.
Free article

Abstract

Background: Levetiracetam is an antiepileptic drug used to prevent or treat seizure in patients with severe traumatic brain injury. This study aimed to develop and validate methodology suitable for measuring levetiracetam concentrations in human plasma and urine. Methods: Plasma or urine (10 μl) samples were spiked with [2H6]-levetiracetam and processed using an acetonitrile precipitation. ESI-LC-MS/MS was employed for analyte detection. Results: The levetiracetam calibration was linear from 0.1 to 50 mg/l in a combined matrix of plasma and urine. Intra- and inter-assay imprecision and accuracy in plasma were <7.7 and 109%, and in urine were <7.9 and 108%, respectively. Conclusion: The validated method was applied to a pharmacokinetic study of levetiracetam in critically ill patients with severe traumatic brain injury.

Keywords: chromatography; levetiracetam; mass spectrometry; pharmacokinetic; traumatic brain injury; triple quadrupole.

Plain language summary

Levetiracetam is a drug that is used for the prevention or treatment of seizure. This study aimed to design a method that would be suitable for measuring levetiracetam in human plasma and urine. The method was subsequently applied to a clinical study of patients with severe traumatic brain injury.

PubMed Disclaimer

MeSH terms

LinkOut - more resources