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. 2023:2629:95-114.
doi: 10.1007/978-1-0716-2986-4_6.

Analysis of Single-Cell RNA-seq Data

Xiaoru Dong  1 , Rhonda Bacher  2
Affiliations

Analysis of Single-Cell RNA-seq Data

Xiaoru Dong et al. Methods Mol Biol. 2023.

Abstract

As single-cell RNA sequencing experiments continue to advance scientific discoveries across biological disciplines, an increasing number of analysis tools and workflows for analyzing the data have been developed. In this chapter, we describe a standard workflow and elaborate on relevant data analysis tools for analyzing single-cell RNA sequencing data. We provide recommendations for the appropriate use of commonly used methods, with code examples and analysis interpretations.

Keywords: Gene expression; High-throughput sequencing; Normalization; Quality control; Single-cell RNA-seq.

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References

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