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. 2023 May;42(5):593-596.
doi: 10.1007/s10096-023-04563-3. Epub 2023 Mar 16.

RIDA®GENE Helicobacter pylori PCR on the ELITe InGenius System

Lucie Bénéjat  1   2 , Astrid Ducournau  1   2 , Chloé Domingues Martins  3 , Emilie Bessède  1   2 , Philippe Lehours  4   5   6
Affiliations

RIDA®GENE Helicobacter pylori PCR on the ELITe InGenius System

Lucie Bénéjat et al. Eur J Clin Microbiol Infect Dis. 2023 May.

Abstract

PCR detection of Helicobacter pylori infection in gastric biopsies allows the detection of this bacterium and the mutations associated with macrolide resistance. The aim of this study was to evaluate the performance of RIDA®GENE H. pylori PCR (r-Biopharm) on the ELITe InGenius System (Elitech). Two hundred gastric biopsies were obtained. These biopsies were ground in nutrient broth. Two hundred microliters of this suspension was treated with proteinase K, and then, 200 µL was transferred to an ELITe InGenius sample tube and tested using RIDA®GENE H. pylori PCR reagents. In-house H. pylori PCR was used as a reference. The sensitivity of RIDA®GENE H. pylori PCR with ELITe InGenius was 100%, the specificity was 98% (95% confidence interval (CI), 95.3-100%), the PPV was 98% (95% CI, 95.3-100%), and the NPV was 100% for the detection of H. pylori. All of these parameters were 100% for the categorization of macrolide resistance. The adaptation of RIDA®GENE H. pylori PCR reagents on the ELITe InGenius System was successful. This PCR is easy to use on this system.

Keywords: Automation; Clarithromycin resistance; ELITe InGenius; H. pylori; PCR.

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Conflict of interest statement

The authors declare no competing interests.

References

    1. Megraud F, Lehours P. Helicobacter pylori detection and antimicrobial susceptibility testing. Clin Microbiol Rev [Internet] 2007;20:280–322. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dop... - PMC - PubMed
    1. Bénéjat L, Ducournau A, Lehours P, Mégraud F. Real-time PCR for Helicobacter pylori diagnosis The best tools available. Helicobacter. 2018;23:12512. doi: 10.1111/hel.12512. - DOI - PubMed
    1. Jehanne Q, Bénéjat L, Mégraud F, Bessède E, Lehours P. Evaluation of the AllplexTM H pylori and ClariR PCR Assay for Helicobacter pylori detection on gastric biopsies. Helicobacter. 2020;25:12702. doi: 10.1111/hel.12702. - DOI - PubMed
    1. Hays C, Delerue T, Lamarque D, Burucoa C, Collobert G, Billöet A, et al. Molecular diagnosis of Helicobacter pylori infection in gastric biopsies: Evaluation of the Amplidiag® H pylori + ClariR assay. Helicobacter. 2019;24:12560. doi: 10.1111/hel.12560. - DOI - PubMed
    1. Berenger BM, Chui L, Ferrato C, Lloyd T, Li V, Pillai DR. Performance of four commercial real-time PCR assays for the detection of bacterial enteric pathogens in clinical samples. Int. J. Infect. Dis. IJID Off. Publ. Int. Soc. Infect. Dis. 2021 23;S1201-9712(21)00831-6. 10.1016/j.ijid.2021.10.035 - PubMed