Toward a biomarker panel measured in CNS-originating extracellular vesicles for improved differential diagnosis of Parkinson's disease and multiple system atrophy

Hash Brown Taha^{1

2}, Simon Hornung^{1

3}, Suman Dutta¹, Leony Fenwick¹, Otmane Lahgui¹, Kathryn Howe¹, Nour Elabed¹, Irish Del Rosario⁴, Darice Y Wong^{1

5}, Aline Duarte Folle⁴, Daniela Markovic⁶, Jose-Alberto Palma⁷, Un Jung Kang⁸, Roy N Alcalay^{9

10}, Miriam Sklerov¹¹, Horacio Kaufmann⁷, Brent L Fogel^{1

5

12}, Jeff M Bronstein^{1

13}, Beate Ritz^{4

13}, Gal Bitan^{14

15

16}

Affiliations

¹ Department of Neurology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, 90095, USA.
² Department of Integrative Biology and Physiology, University of California Los Angeles, Los Angeles, CA, 90095, USA.
³ Division of Peptide Biochemistry, TUM School of Life Sciences, Technical University of Munich, 85354, Freising, Germany.
⁴ Department of Epidemiology, Fielding School of Public Health, University of California Los Angeles, Los Angeles, CA, 90095, USA.
⁵ Department of Human Genetics, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, 90095, USA.
⁶ Department of Medicine Statistics Core, Division of General Internal Medicine and Health Services Research, University of California Los Angeles, Los Angeles, CA, 90095, USA.
⁷ Department of Neurology, Dysautonomia Center, New York University School of Medicine, New York, NY, 10016, USA.
⁸ Department of Neurology, The Marlene and Paolo Fresco Institute for Parkinson's and Movement Disorders, New York University School of Medicine, New York, NY, 10016, USA.
⁹ Department of Neurology, Taub Institute for Research on Alzheimer's Disease and the Aging Brain, Columbia University, New York, NY, 10032, USA.
¹⁰ Department of Neurology, Tel Aviv Sourasky Medical Center, Tel Aviv, Israel.
¹¹ Department of Neurology, University of North Carolina School of Medicine, Chapel Hill, NC, 27599, USA.
¹² UCLA Clinical Neurogenomics Research Center, David Geffen School of Medicine, University of California, Los Angeles, CA, 90095, USA.
¹³ Brain Research Institute, University of California, Los Angeles, CA, 90095, USA.
¹⁴ Department of Neurology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, 90095, USA. gbitan@mednet.ucla.edu.
¹⁵ Brain Research Institute, University of California, Los Angeles, CA, 90095, USA. gbitan@mednet.ucla.edu.
¹⁶ Molecular Biology Institute, University of California Los Angeles, Los Angeles, CA, 90095, USA. gbitan@mednet.ucla.edu.

PMID: 36935518
PMCID: PMC10026428
DOI: 10.1186/s40035-023-00346-0

Toward a biomarker panel measured in CNS-originating extracellular vesicles for improved differential diagnosis of Parkinson's disease and multiple system atrophy

Hash Brown Taha et al. Transl Neurodegener. 2023.

. 2023 Mar 20;12(1):14.

doi: 10.1186/s40035-023-00346-0.

Authors

Affiliations

¹ Department of Neurology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, 90095, USA.
² Department of Integrative Biology and Physiology, University of California Los Angeles, Los Angeles, CA, 90095, USA.
³ Division of Peptide Biochemistry, TUM School of Life Sciences, Technical University of Munich, 85354, Freising, Germany.
⁴ Department of Epidemiology, Fielding School of Public Health, University of California Los Angeles, Los Angeles, CA, 90095, USA.
⁵ Department of Human Genetics, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, 90095, USA.
⁶ Department of Medicine Statistics Core, Division of General Internal Medicine and Health Services Research, University of California Los Angeles, Los Angeles, CA, 90095, USA.
⁷ Department of Neurology, Dysautonomia Center, New York University School of Medicine, New York, NY, 10016, USA.
⁸ Department of Neurology, The Marlene and Paolo Fresco Institute for Parkinson's and Movement Disorders, New York University School of Medicine, New York, NY, 10016, USA.
⁹ Department of Neurology, Taub Institute for Research on Alzheimer's Disease and the Aging Brain, Columbia University, New York, NY, 10032, USA.
¹⁰ Department of Neurology, Tel Aviv Sourasky Medical Center, Tel Aviv, Israel.
¹¹ Department of Neurology, University of North Carolina School of Medicine, Chapel Hill, NC, 27599, USA.
¹² UCLA Clinical Neurogenomics Research Center, David Geffen School of Medicine, University of California, Los Angeles, CA, 90095, USA.
¹³ Brain Research Institute, University of California, Los Angeles, CA, 90095, USA.
¹⁴ Department of Neurology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA, 90095, USA. gbitan@mednet.ucla.edu.
¹⁵ Brain Research Institute, University of California, Los Angeles, CA, 90095, USA. gbitan@mednet.ucla.edu.
¹⁶ Molecular Biology Institute, University of California Los Angeles, Los Angeles, CA, 90095, USA. gbitan@mednet.ucla.edu.

PMID: 36935518
PMCID: PMC10026428
DOI: 10.1186/s40035-023-00346-0

No abstract available

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

**Fig. 1**
Biomarker analysis in HC, PD, and MSA. a, b pS129-α-Syn and Tau concentrations were measured using ECLIA, log-transformed, and analyzed by a mixed-effect analysis with post-hoc Tukey test. Data are represented as mean ± SD. Values below the lower limit of detection were imputed as the minimum value divided by 2. c NfL concentrations were measured using Simoa, log-transformed, and analyzed by a one-way ANOVA with post-hoc Tukey test. Data are represented as mean ± SD. d, e ROC analyses of biomarker concentrations using a multinomial logistic regression model with LASSO variable selection (Additional file 1: Fig. S3). The model included nEV α-syn concentration, oEV:nEV α-syn concentration ratio, total EV concentration in the sample and d oEV pS129-α-syn or e oEV tau concentration. The presented models do not include NfL

See this image and copyright information in PMC

References

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Toward a biomarker panel measured in CNS-originating extracellular vesicles for improved differential diagnosis of Parkinson's disease and multiple system atrophy

Affiliations

Toward a biomarker panel measured in CNS-originating extracellular vesicles for improved differential diagnosis of Parkinson's disease and multiple system atrophy

Authors

Affiliations

Conflict of interest statement

Figures

References

Publication types

MeSH terms

Substances

LinkOut - more resources

Full Text Sources

Medical