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. 2023 Mar 7;25(4):160.
doi: 10.3892/ol.2023.13746. eCollection 2023 Apr.

Low expression of SEMA4D as a potential predictive molecular marker of poor survival in patients with melanoma combined with liver cancer

Xiang Liu  1 , Chong Zhang  1 , Wu-Han Yang  1 , Sheng-Chao Li  1 , Rui-Feng Wang  2 , Yi-Bin Zhang  2 , Zhi-Lei Zhang  1
Affiliations

Low expression of SEMA4D as a potential predictive molecular marker of poor survival in patients with melanoma combined with liver cancer

Xiang Liu et al. Oncol Lett. .

Abstract

This study explored the correlation between semaphorin 4D (SEMA4D) and the prognosis and survival time of patients with melanoma combined with liver cancer. A total of 272 patients were recruited, and clinical and follow-up data were recorded. The expression levels of SEMA4D and SEMA3B were determined. Pearson's χ2 test and Spearman's rank correlation coefficient were used to analyze the relationship between prognosis and the assessed parameters of melanoma patients. Univariate and multivariate Logistic regression and Cox proportional risk regression analyses were used for further analysis. Additionally, receiver operating characteristic curve and survival curves of subjects were plotted. The Pearson's χ2 test showed that the prognosis of melanoma patients was significantly correlated with age, tumor grade, and decreased SEMA4D expression. Additionally, Spearman's correlation coefficient analysis showed that age, tumor grade, and SEMA4D expression were significantly correlated with prognosis. Univariate logistic regression analysis showed that age and tumor grade, and SEMA4D expression, were significantly correlated with prognosis. Older patients, a higher tumor grade, and lower SEMA4D expression were associated with a poorer prognosis. Multivariate logistic regression analysis showed that older patients had a poorer prognosis, and patients with lower SEMA4D expression levels had a significantly worse prognosis than patients with higher SEMA4D expression levels. Kaplan-Meier analysis showed that the survival time of older patients was lower than that of the younger patients. The survival times of patients with lower SEMA4D expression levels were significantly lower than that of patients with higher SEMA4D expression levels. Multivariate Cox regression analysis showed that the survival time of older patients was lower than that of younger patients. The survival time of melanoma patients with low SEMA4D expression was significantly lower than that of patients with higher SEMA4D expression. SEMA4D was significantly associated with melanoma, and lower SEMA4D expression was associated with a poorer survival prognosis in melanoma patients.

Keywords: melanoma; prognosis; semaphorin 4D; survival time.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Figure 1.
Figure 1.
Comparison of SEMA4D expression between melanoma and normal tissues. *P<0.05. Purple, melanoma; Blue, normal. SEMA4D, semaphorin 4D. SKCM, skin cutaneous melanoma; T, tumor; N, normal.
Figure 2.
Figure 2.
ROC curves. ROC curves of (A) Sex, (B) Age, (C) Tumor size, (D) Family history, (E) Tumor grade, (F) SEMA4D, and (G) Tumor stage. SEMA4D, semaphorin 4D; ROC, receiver operating characteristic; AUC, area under the curve.
Figure 3.
Figure 3.
Receiver operating characteristic curve of all relevant factors for melanoma. SEMA4D, semaphorin 4D.
Figure 4.
Figure 4.
Receiver operating characteristic curve of the combined influence of all patient-related factors on melanoma. AUC, area under the curve.
Figure 5.
Figure 5.
Survival curve analysis. Survival curve based on (A) Sex, (B) Age, (C) Tumor size, (D) Family history, (E) Tumor grade, (F) SEMA4D, and (G) Tumor stage. SEMA4D, semaphorin 4D; HR, hazard ratio; CI, confidence interval.
Figure 6.
Figure 6.
Expression of SEMA4D in liver cancer tissues. (A) mRNA expression of SEMA4D. (B) Protein expression levels of SEMA4D and SEMA3B. SEMA, semaphoring; CON, control.
See this image and copyright information in PMC

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