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. 2023 Mar 1:13:1089919.
doi: 10.3389/fcimb.2023.1089919. eCollection 2023.

Optimization and standardization of mNGS-based procedures for the diagnosis of Mycoplasma periprosthetic joint infection: A novel diagnostic strategy for rare bacterial periprosthetic joint infection

Yuanqing Cai  1   2   3 , Haiqi Ding  1   3 , Xiaoqing Chen  4 , Yang Chen  1   3 , Changyu Huang  1   3 , Chaofan Zhang  1   3 , Zida Huang  1   3 , Ying Huang  1   3 , Wenbo Li  1   3 , Wenming Zhang  1   3 , Xinyu Fang  1   3
Affiliations

Optimization and standardization of mNGS-based procedures for the diagnosis of Mycoplasma periprosthetic joint infection: A novel diagnostic strategy for rare bacterial periprosthetic joint infection

Yuanqing Cai et al. Front Cell Infect Microbiol. .

Abstract

Introduction: The diagnosis of Mycoplasma periprosthetic joint infection (PJI) is rather difficult due to its rarity and difficult in isolation, there are not standardized diagnostic procedure for Mycoplasma PJI presently. This study aimed to reported a metagenomic next-generation sequencing (mNGS)-based diagnostic strategy for Mycoplasma PJI.

Methods: In the present study, we have reported the largest number of Mycoplasma PJI that were precisely diagnosed by mNGS and verified by optimized microbial culture methods and (or) 16S PCR polymerase chain reaction (PCR).

Results: The positive rate of optimized microbial culture methods and 16S PCR in the detection of Mycoplasma PJI was 57.14% and 71.43%, respectively. The infections were well controlled by targeted treatment in all cases.

Conclusion: The standardized and optimized procedure based on mNGS presented in this study is useful for the diagnosis of Mycoplasma PJI, which might also be provided as a novel diagnostic strategy for rare bacterial PJI.

Keywords: Mycoplasma; metagenomic next-generation sequencing; periprosthetic joint infection; procedure; rare bacteria.

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Figures

Figure 1
Figure 1
The clinical data of case 5. (A) the imaging data of case 4; (B) there was a sinus tract behind the knee; (C) purulent synovial fluid was obtained from preoperative aspiration; (D) mNGS indicated Mycoplasma hominis infection; (E) intraoperative pathological test showed there were a large amount of neutrophils in periprosthetic tissues.
Figure 2
Figure 2
The optimized and standardized procedure based on mNGS for Mycoplasma PJI. mNGS, metagenomic next-generation sequencing; PCR, polymerase chain reaction; PJI, periprosthetic joint infection; -, microbial culture negative; +, microbial culture positive. Parts of the figure were drawn by using pictures from Servier Medical Art. Servier Medical Art by Servier is licensed under a Creative Commons Attribution 3.0 Unported License (https://creativecommons.org/licenses/by/3.0/).
See this image and copyright information in PMC

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