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. 1987 Dec 24;105(2):183-92.
doi: 10.1016/0022-1759(87)90265-1.

Studies of serum-free culture medium in the generation of lymphokine activated killer cells

L M Muul  1 , K Nason-Burchenal, C Hyatt, S Schwarz, D Slavin, E P Director, S A Rosenberg
Affiliations

Studies of serum-free culture medium in the generation of lymphokine activated killer cells

L M Muul et al. J Immunol Methods. .

Abstract

Lymphokine activated killer (LAK) cells administered in conjunction with recombinant interleukin-2 can mediate the regression of metastatic tumor in some patients with advanced cancer. In these trials LAK cells were activated in medium containing 2% human type A or AB serum. We have found three commercially available, serum-free culture media which allow development of in vitro LAK activity by human peripheral blood lymphocytes. They are AIMV (Gibco), MASF-3 (Whitaker-MA Bioproducts) and HB-104 (Dupont). If 2-mercaptoethanol was added to these culture media they were also capable of generating murine LAK cells which were effective in reducing pulmonary metastases in the murine MCA-106 model. Although LAK cells generated in these media have not been tested in humans yet, potentially they could provide a safe, unlimited and less expensive source of culture fluid for generating the large numbers of LAK cells needed for human clinical trials.

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