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. 2023 Mar;44(3):246-252.
doi: 10.15537/smj.2023.44.3.20220768.

Efficacy of resveratrol against breast cancer and hepatocellular carcinoma cell lines

Nouf A ALkharashi  1
Affiliations

Efficacy of resveratrol against breast cancer and hepatocellular carcinoma cell lines

Nouf A ALkharashi. Saudi Med J. 2023 Mar.

Abstract

Objectives: To evaluate the anti-cancer effect of resveratrol on Michigan cancer foundation-7 (MCF-7) and hepatoblastoma cell line (HepG2) cells.

Methods: The study was carried out at the Department of Botany and Microbiology, Prince Sattam bin Abdulaziz University, Al-kharj, Saudi Arabia, from August 2022 to October 2022. Different concentrations of resveratrol were added to the MCF-7 and HepG2 cell lines. Cell death and proliferation were measured with MTT and Trypan blue exclusion assays. Apoptosis markers were assessed by using a quantitative PCR assay (qPCR).

Results: The resveratrol was shown to suppress the proliferation of MCF-7 and HepG2 cells at dose- and time-dependent. The cytotoxic effect of resveratrol was observed even at 100 μM after 24 hours. In comparison to untreated cells, resveratrol treatment reduced the viability of MCF-7 cells to roughly 57.5% with a half maximal inhibitory concentration (IC50) of 51.18 μM and HepG2 cells to 56.2% with an IC50 of 57.4 μM. Furthermore, in the tested cell lines, resveratrol was able to induce apoptosis mediated by elevated apoptosis markers.

Conclusion: Resveratrol appears to be an excellent candidate agent in anticancer therapy in various human cancers.

Keywords: HepG2 cell line; MCF-7; MTT; proliferation; resveratrol.

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Figures

Figure 1
Figure 1
- Cytotoxicity assessment by MTT assay in MCF-7 and HepG2 cell lines treated with varius concentrations of resveratrol for 24 hours. ** p<0.01
Figure 2
Figure 2
- The percentage of MCF-7 and HepG2 cells viability using a Trypan blue extraction assay for 24 hours exposure with different concentrations of resveratrol. ** p<0.01
Figure 3
Figure 3
- The mRNA levels of caspase-3, caspase-8, and caspase-9 in MCF-7 and HepG2 cells. ** p<0.01
Figure 4
Figure 4
- The mRNA levels of apoptotic markers (Bax, Bcl-2, and Bcl-xL) in MCF-7 and HepG2 cells. ** p<0.01
Figure 5
Figure 5
- The mRNA levels of apoptotic markers of p53 and p21 in MCF-7 and HepG2. ** p<0.01
See this image and copyright information in PMC

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