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. 2023 Sep;43(9):1332-1346.
doi: 10.1002/jat.4468. Epub 2023 Apr 2.

A 90-day toxicity study of tripeptide, arginine-alanine-lysine

Maureen Dunn  1 , Róbert Glávits  2 , Timothy S Murbach  1 , Vickie Modica  1 , John R Endres  1 , Gábor Hirka  2 , Ilona Pasics Szakonyiné  2
Affiliations

A 90-day toxicity study of tripeptide, arginine-alanine-lysine

Maureen Dunn et al. J Appl Toxicol. 2023 Sep.

Abstract

There is a growing global interest in using peptides in the health industry for pharmaceuticals, cosmetics, and natural food products. Peptides contain two or more linked amino acids, whereas more than 50 amino acids are classified as polypeptides. Although there is a growing level of interest in the use of peptides in the health and wellness industry, there is a lack of literature pertaining to a specific tripeptide derived from arginine, alanine, and lysine (RAK) that is of interest for human dietary use. Therefore, a 90-day repeated-dose toxicity study was performed in rats to evaluate the subchronic oral toxicity of RAK. Eighty Han:WIST rats were administered RAK by gavage at doses of 0, 250, 500, or 1000 mg/kg bw/day. There were no mortalities or other treatment related effects, and no target organs were identified. A no-observed-adverse-effect-level (NOAEL) of 1000 mg/kg bw/day, the highest dose tested, was determined. This study will contribute to the body of research in regard to the safety of the use of RAK.

Keywords: NOAEL; RAK; alanine; arginine; lysine; peptide; safety; toxicity; tripeptide.

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References

REFERENCES

    1. Adibi, S. A. (2003). Regulation of expression of the intestinal oligopeptide transporter (Pept-1) in health and disease. American Journal of Physiology. Gastrointestinal and Liver Physiology, 285(5), G779-G788. https://doi.org/10.1152/ajpgi.00056.2003
    1. Chow, F., Dysart, M., Hamar, D., Lewis, L., & Udall, R. (1976). Alanine: a toxicity study. Toxicology and Applied Pharmacology, 37, 491-497. https://doi.org/10.1016/0041-008X(76)90211-8
    1. Czajka, A., Kania, E. M., Genovese, L., Corbo, A., Merone, G., Luci, C., & Sibilla, S. (2018). Daily oral supplementation with collagen peptides combined with vitamins and other bioactive compounds improves skin elasticity and has a beneficial effect on joint and general wellbeing. Nutrition Research, 57, 97-108. https://doi.org/10.1016/j.nutres.2018.06.001
    1. El-Deen, A. E.-S. N., Mansour, A. E.-M., & Taha, A. (2018). High protein diet that cause weight loss and lower blood glucose level have a serious impact on the kidney functions of male diabetic obese albino rats. Food and Nutrition Sciences, 09(10), 1174-1191. https://doi.org/10.4236/fns.2018.910085
    1. Frazier, K., & Seely, J. (2013). Urinary system. In Toxicologic pathology. Nonclinical safety assessment (pp. 421-484). CRC Press.

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