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. 2023 Mar 22;13(1):4699.
doi: 10.1038/s41598-023-31937-6.

Characterization and investigation of electrochemical and biological properties of antibacterial silver nanoparticle-deposited TiO2 nanotube array surfaces

Salih Durdu  1 , Emine Yalçin  2 , Atilgan Altinkök  3 , Kültiğin Çavuşoğlu  4
Affiliations

Characterization and investigation of electrochemical and biological properties of antibacterial silver nanoparticle-deposited TiO2 nanotube array surfaces

Salih Durdu et al. Sci Rep. .

Abstract

The one of main reasons of the premature failure of Ti-based implants is infections. The metal- and metal oxide-based nanoparticles have very high potential on controlling of infections. In this work, the randomly distributed AgNPs-deposited onto well-ordered TiO2 nanotube surfaces were fabricated on titanium by anodic oxidation (AO) and electrochemical deposition (ED) processes. AgNPs-deposited nanotube surfaces, which is beneficial for bone tissue growth exhibited hydrophilic behaviors. Moreover, the AgNPs-deposited nanotube surfaces, which prevent the leaching of metallic Ti ions from the implant surface, indicated great corrosion resistance under SBF conditions. The electrochemical corrosion resistance of AgNPs-deposited nanotube surfaces was improved up to about 145% compared to bare Gr2 surface. The cell viability of AgNPs-deposited nanotube surfaces was improved. Importantly, the AgNPs-deposited nanotube surfaces exhibited antibacterial activity for Gram-positive and Gram-negative bacteria. Eventually, it can be concluded that the AgNPs-deposited nanotube surfaces possess high stability for long-term usage of implant applications.

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Conflict of interest statement

The authors declare no competing interests.

Figures

Figure 1
Figure 1
Surface morphologies of bare nanotube and AgNPs-deposited on nanotube surfaces at different times by ED process: (a) bare nanotube (50,000×), (b) 0.5 min, (c) 1 min and (d) 5 min.
Figure 2
Figure 2
Elemental mapping images of AgNPs-deposited nanotube surfaces for 5 min.
Figure 3
Figure 3
XRD spectra of AgNPs-deposited (Gr2-50 V-Ag-5 m) nanotube surfaces.
Figure 4
Figure 4
Contact angle images of the surfaces: (a) bare Gr2, (b) bare TiO2 nanotube, (c) Gr2-50 V-Ag-0.5 m, (d) Gr2-50 V-Ag-1 m and (e) Gr2-50 V-Ag-5 m.
Figure 5
Figure 5
Tafel polarization curves of bare Gr2, bare nanotube and AgNPs-deposited nanotube surfaces.
Figure 6
Figure 6
MTT test results of bare Gr2 and AgNPs-deposited nanotube surfaces. Different letters shown in the graph indicate statistical significance (p < 0.05).
Figure 7
Figure 7
ALP activity in cells adhering to AgNPs-deposited nanotube surfaces.
Figure 8
Figure 8
Percentage of bacterial inhibition of AgNPs-deposited nanotube surfaces.
Figure 9
Figure 9
Reduction in bacterial colonies after re-culturation in samples with the highest antibacterial activity on AgNPs-deposited surfaces (A) S. typhimurium viability after re-culture on bare Gr2 surfaces, (B) S. typhimurium viability after re-culture on Gr2-50 V-Ag-5 m surfaces.
See this image and copyright information in PMC

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