Integration of transcriptome and proteome analyses reveals the regulation mechanisms of Larimichthys polyactis liver exposed to heat stress

Abstract

Small yellow croaker (Larimichthys polyactis) is one of the most economically important marine fishery species. L. polyactis aquaculture has experienced stress response and the frequent occurrence of diseases, bringing huge losses to the aquaculture industry. Little is known about the regulation mechanism of heat stress response in L. polyactis. In this study, to provide an overview of the heat-tolerance mechanism of L. polyactis, the transcriptome and proteome of the liver of L. polyactis on the 6 h after high temperature (32 °C) treatment were analyzed using Illumina HiSeq 4000 platform and isobaric tag for relative and absolute quantitation (iTRAQ). A total of 3700 upregulated and 1628 downregulated genes (differentially expressed genes, DEGs) were identified after heat stress in L. polyactis. Also, 198 differentially expressed proteins (DEPs), including 117 upregulated and 81 downregulated proteins, were identified. Integrative analysis revealed that 72 genes were significantly differentially expressed at transcriptome and protein levels. Functional analysis showed that arginine biosynthesis, tyrosine metabolism, pentose phosphate pathway, starch and sucrose metabolism, and protein processing in the endoplasmic reticulum were the main pathways responding to heat stress. Among the pathways, protein processing in the endoplasmic reticulum was enriched by most DEGs/DEPs, which suggests that this pathway may play a more important role in the heat stress response. Further insights into the pathway revealed that transcripts and proteins, especially HSPs and PDIs, were differentially expressed in response to heat stress. These findings contribute to existing data describing the fish response to heat stress and provide information about protein levels, which are of great significance to a deeper understanding of the heat stress responding regulation mechanism in L. polyactis and other fish species.

Keywords: Heat stress; Larimichthys polyactis; Liver response; Proteomic analysis; Transcriptomic analysis.