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. 2023 May 15:669:115119.
doi: 10.1016/j.ab.2023.115119. Epub 2023 Mar 21.

Increased lentivirus titer using ultra expression vectors

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Increased lentivirus titer using ultra expression vectors

So Fujibayashi et al. Anal Biochem. .

Abstract

Lentivirus is an efficient gene transfer system that is widely used in basic science. We aimed to improve viral titer by applying an ultra-expression vectors to lentiviral packaging. Application of the ultra-expression vectors increased biological titer 4 times for standard preparation. We also evaluated the efficacy of the ultra-expression vectors to relatively longer insert fragments, such as CSII-CMV-CNROE containing 5 genes in multiple cloning sites. Packaging of the ultra-expression vectors showed 3.5 times higher biological titer compared with the original method. Our improved packaging system could be applied to lentivirus to produce higher titers.

Keywords: Lentivirus; Packaging system; Transcriptional factors; Ultra-expression vectors.

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Conflict of interest statement

Declaration of competing interest All authors do not have any conflict of interest and competing interest to disclose.

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