Increased lentivirus titer using ultra expression vectors
- PMID: 36958509
- DOI: 10.1016/j.ab.2023.115119
Increased lentivirus titer using ultra expression vectors
Abstract
Lentivirus is an efficient gene transfer system that is widely used in basic science. We aimed to improve viral titer by applying an ultra-expression vectors to lentiviral packaging. Application of the ultra-expression vectors increased biological titer 4 times for standard preparation. We also evaluated the efficacy of the ultra-expression vectors to relatively longer insert fragments, such as CSII-CMV-CNROE containing 5 genes in multiple cloning sites. Packaging of the ultra-expression vectors showed 3.5 times higher biological titer compared with the original method. Our improved packaging system could be applied to lentivirus to produce higher titers.
Keywords: Lentivirus; Packaging system; Transcriptional factors; Ultra-expression vectors.
Copyright © 2023 Elsevier Inc. All rights reserved.
Conflict of interest statement
Declaration of competing interest All authors do not have any conflict of interest and competing interest to disclose.
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