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Comment
. 2023 Apr;9(4):511-512.
doi: 10.1038/s41477-023-01388-y.

A genome-scale CRISPR tool for targeting multiple gene family members at once

No authors listed
Comment

A genome-scale CRISPR tool for targeting multiple gene family members at once

No authors listed. Nat Plants. 2023 Apr.
No abstract available

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References

    1. Li, Z. et al. Gene duplicability of core genes is highly consistent across all angiosperms. Plant Cell 28, 326–344 (2015). A large-scale study of duplicate retention for 9,178 gene families shared over 37 flowering plant species; a consistent pattern of gene duplicability was observed across species. - DOI
    1. Rensing, S. A. Gene duplication as a driver of plant morphogenetic evolution. Curr. Opin. Plant Biol. 17, 43–48 (2014). A review article proposing that plants evolve developmental novelties by retaining duplicate copies of control genes.
    1. Grützner, R. et al. High-efficiency genome editing in plants mediated by a Cas9 gene containing multiple introns. Plant Commun. 2, 1–15 (2021). This paper reveals that adding 13 introns into the Cas9 coding sequence improves its efficiency in generating mutations in target genes. - DOI
    1. Gaillochet, C., Develtere, W. & Jacobs, T. B. CRISPR screens in plants: Approaches, guidelines, and future prospects. Plant Cell 33, 794–813 (2021). A review article presenting the general concepts, tools, and workflows for establishing CRISPR screens in plants.
    1. Pan, C. et al. Guide RNA library-based CRISPR screens in plants: opportunities and challenges. Curr. Opin. Biotech. 79, 102883 (2023). A review article on sgRNA library-based CRISPR screens in plants.

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