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. 2023 Mar 8;13(3):728.
doi: 10.3390/life13030728.

In Vitro Pharmacological Activity, and Comparison GC-ToF-MS Profiling of Extracts from Cissus cornifolia (Baker) Planch

Nkoana I Mongalo  1 , Maropeng Vellry Raletsena  2 , Rabelani Munyai  2
Affiliations

In Vitro Pharmacological Activity, and Comparison GC-ToF-MS Profiling of Extracts from Cissus cornifolia (Baker) Planch

Nkoana I Mongalo et al. Life (Basel). .

Abstract

Cissus cornifolia (Baker) Planch is traditionally used in South African traditional medicine (SATM) to treat a variety of human infections. The antimicrobial activity of extracts from C. cornifolia was investigated in vitro against a plethora of pathogenic microorganisms using the microdilution assay. The acetone extract exhibited a notable minimum inhibitory concentration (MIC) value of 0.10 mg/mL against Mycoplasma hominis and a further MIC of 0.20 mg/mL against Candida parapsilosis, Streptococcus agalactiae, Pseudomonas aeruginosa, and Enterococcus faecalis. In the antiproliferative assays, both the ethyl acetate and methanol extracts exhibited a potent inhibition of the MCF-7-21 cell line. In the anti-inflammatory assays, both the ethyl acetate and methanol extracts exhibited IC50 values of 15.59 and 15.78 µg/mL against Cyclooxygenase-2 (COX-2), respectively. Methanol extract further exhibited potent dual inhibition of both COX-2 and 15-LOX enzymes, hence, recommended to curb both related cancers, particularly breast cancer and inflammation-borne diseases. In the comparative gas chromatography time-of-flight mass spectrometry (GC/TOF-MS), the acetone, ethyl acetate, and methanol extract contained significantly prevalent amounts of compound 2-(2',4',4',6',6',8',8'-Heptamethyltetrasiloxan-2'-yloxy)-2,4,4,6,6,8,8,10,10-nonamethylcyclopentasiloxane with % area ranging from 15.714 to 39.225. The findings in the current work validates the use of the plant species in SATM in the treatment of cancer-like infections, opportunistic infections associated with HIV-AIDS. Furthermore, the in vivo studies and the mechanisms of action still need to be explored.

Keywords: Cissus cornifolia; anti-inflammatory; antimicrobial activity; antiproliferative; gas chromatography time-of-flight mass spectrometry (GC-ToF-MS).

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
The total activity (mL/g) of various Cissus cornifolia extracts against selected microorganisms.
See this image and copyright information in PMC

References

    1. Raimondo D., Von Staden L., Foden W., Victor J.E., Helme N.A., Turner R.C., Kamundi D.A., Manyama P.A. Red List of South African Plants, Strelitzia 25. South African National Biodiversity Institute; Pretoria, South Africa: 2009.
    1. Perovic O., Chetty V., Iyaloo S. Antimicrobial resistance surveillance from sentinel public hospitals, South Africa. Comm. Dis. Surv. Bull. 2014;13:1–12.
    1. Padmapriyadarsini C., Narendran G., Soumya Swaminathan S. Diagnosis, and treatment of tuberculosis in HIV co-infected patients. Ind. J. Med. Res. 2011;134:850–865. - PMC - PubMed
    1. Kichu M., Malewska T., Akter K., Imchen I., Harrington D., Kohen J., Vemulpad S.R., Jamie J.F. An ethnobotanical study of medicinal plants of Chungtia village, Nagaland, India. J. Ethnopharmacol. 2015;166:5–17. doi: 10.1016/j.jep.2015.02.053. - DOI - PubMed
    1. Jayachitra C., Jamuna S., Ali M.A., Paulsamy S., Al-Hemaid F.M.A. Evaluation of traditional medicinal plant, Cissus setosa Roxb. (Vitaceae) for the antiulcer property. Saudi J. Biol. Sci. 2018;25:293–297. doi: 10.1016/j.sjbs.2017.03.007. - DOI - PMC - PubMed

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