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. 2023 Feb 27;11(3):595.
doi: 10.3390/microorganisms11030595.

Fish Probiotics: Cell Surface Properties of Fish Intestinal Lactobacilli and Escherichia coli

Susanna Mirzabekyan  1 , Natalya Harutyunyan  1 , Anahit Manvelyan  1 , Lilit Malkhasyan  1 , Marine Balayan  1 , Shakhlo Miralimova  2 , Michael L Chikindas  3   4   5 , Vladimir Chistyakov  4   6 , Astghik Pepoyan  1   7
Affiliations

Fish Probiotics: Cell Surface Properties of Fish Intestinal Lactobacilli and Escherichia coli

Susanna Mirzabekyan et al. Microorganisms. .

Abstract

The properties of intestinal bacteria/probiotics, such as cell surface hydrophobicity (CSH), auto-aggregation, and biofilm formation ability, play an important role in shaping the relationship between the bacteria and the host. The current study aimed to investigate the cell surface properties of fish intestinal bacteria and probiotics. Microbial adhesion to hydrocarbons was tested according to Kos and coauthors. The aggregation abilities of the investigated strains were studied as described by Collado and coauthors. The ability of bacterial isolates to form a biofilm was determined by performing a qualitative analysis using crystal violet staining based on the attachment of bacteria to polystyrene. These studies prove that bacterial cell surface hydrophobicity (CSH) is associated with the growth medium, and the effect of the growth medium on CSH is species-specific and likely also strain-specific. Isolates of intestinal lactobacilli from fish (Salmo ischchan) differed from isolates of non-fish/shrimp origin in the relationship between auto-aggregation and biofilm formation. Average CSH levels for fish lactobacilli and E. coli might were lower compared to those of non-fish origin, which may affect the efficiency of non-fish probiotics use in fisheries due to the peculiarities of the hosts' aquatic lifestyles.

Keywords: E. coli; auto-aggregation; biofilm formation ability; cell surface hydrophobicity; fish; lactic acid bacteria; probiotic.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

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