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. 2022 Nov;11(11):7335-7338.
doi: 10.4103/jfmpc.jfmpc_1211_21. Epub 2022 Dec 16.

Utility of Flow Cytometry in Diagnosis of Acute Leukemias: A Study at RIMS, Ranchi, India

Sunil K Mahto  1 , Niraj Prasad  1   2
Affiliations

Utility of Flow Cytometry in Diagnosis of Acute Leukemias: A Study at RIMS, Ranchi, India

Sunil K Mahto et al. J Family Med Prim Care. 2022 Nov.

Abstract

Introduction: Leukemia is a neoplastic disorder originating in a hematopoietic cell that has undergone an intrinsic change, causing it to escape from the normal restraints imposed on proliferative activity. Immunophenotyping is now the preferred method for diagnosing, classifying, staging and monitoring the disease progression as well as response to therapy.

Material and method: The material of the present study consisted of 51 patients suffering from hematological malignancies who attended and /or were admitted in Rajendra Institute of Medical Sciences, Ranchi during the period from March 2018 to August 2019.

Results: A total of 51 cases were diagnosed as acute leukemia on microscopic examination. On immunophenotyping, 36 cases (70.6%) were diagnosed as Acute Myeloid Leukemia (AML), 15 cases (29.4%) were diagnosed as Acute Lymphoblastic Leukemia (ALL). ALL cases were further divided into B-Cell ALL and T-Cell ALL with 8 cases (15.7%) and 7 cases (13.7%) respectively. Cytogenetics could not be done for these cases due to non-availability of the set-up for the same at the institute.

Conclusion: Flowcytometry can be a great tool in diagnosis and categorisation of leukemia especially at centres where cytogenetics is not available.

Keywords: Flow cytometry; immunophenotyping; leukemia.

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Conflict of interest statement

There are no conflicts of interest.

Figures

Figure 1
Figure 1
Pie chart showing patients with AML and ALL (original image)
Figure 2
Figure 2
Graphs showing positivity for cMPO, CD10, CD13, CD33, and CD117 cCD3 (original image)
Figure 3
Figure 3
Micrograph showing bone marrow picture of Acute Myeloid Leukaemia (100× magnification) (original image)
Figure 4
Figure 4
Micrograph showing Myeloblast with Auer rods (100× magnification) (original image)
Figure 5
Figure 5
Micrograph showing bone marrow picture of Acute lymphoblastic leukemia (100× magnification) (original image)
See this image and copyright information in PMC

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