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. 2023:2653:39-52.
doi: 10.1007/978-1-0716-3131-7_3.

CRISPR/LbCas12a-Mediated Genome Editing in Soybean

Dawei Liang  1 , Yubo Liu  2 , Chao Li  2 , Qin Wen  2 , Jianping Xu  2 , Lizhao Geng  2 , Chunxia Liu  2 , Huaibing Jin  2 , Yang Gao  2 , Heng Zhong  3 , John Dawson  3 , Bin Tian  3 , Brenden Barco  3 , Xiujuan Su  3 , Shujie Dong  3 , Changbao Li  3 , Sivamani Elumalai  3 , Qiudeng Que  4 , Ian Jepson  3 , Liang Shi  3
Affiliations

CRISPR/LbCas12a-Mediated Genome Editing in Soybean

Dawei Liang et al. Methods Mol Biol. 2023.

Abstract

Currently methods for generating soybean edited lines are time-consuming, inefficient, and limited to certain genotypes. Here we describe a fast and highly efficient genome editing method based on CRISPR-Cas12a nuclease system in soybean. The method uses Agrobacterium-mediated transformation to deliver editing constructs and uses aadA or ALS genes as selectable marker. It only takes about 45 days to obtain greenhouse-ready edited plants at higher than 30% transformation efficiency and 50% editing rate. The method is applicable to other selectable markers including EPSPS and has low transgene chimera rate. The method is also genotype-flexible and has been applied to genome editing of several elite soybean varieties.

Keywords: CRISPR-LbCas12a; Fast transformation; Genotype-independent transformation; Plant selectable marker gene; Soybean genome editing; Soybean transformation.

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