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. 2023 Jun 1:239:124198.
doi: 10.1016/j.ijbiomac.2023.124198. Epub 2023 Mar 28.

Fabrication and characterization of colorimetric indicator for Salmon freshness monitoring using agar/polyvinyl alcohol gel and anthocyanin from different plant sources

Huang Xiaowei  1 , Du Liuzi  2 , Li Zhihua  3 , Xue Jin  2 , Shi Jiyong  1 , Zhai Xiaodong  2 , Zhang Junjun  2 , Zhang Ning  2 , Melvin Holmes  4 , Zou Xiaobo  5
Affiliations

Fabrication and characterization of colorimetric indicator for Salmon freshness monitoring using agar/polyvinyl alcohol gel and anthocyanin from different plant sources

Huang Xiaowei et al. Int J Biol Macromol. .

Abstract

In order to investigate the sensitivity of anthocyanins from different plant origin as indicators for salmon freshness, nine plant anthocyanins were extracted and fabricated into colorimetric sensor arrays to detect NH3, trimethylamine (TMA), dimethylamine (DMA) to indicate salmon freshness. Rosella anthocyanin had the highest sensitivity for amines, ammonia and salmon. HPLC-MSS analysis indicated that Delphinidin-3 glucoside accounted for 75.48 % of the Rosella anthocyanin. UV-visible spectral analysis showed that the maximum absorbance band of Roselle anthocyanins for acid and alkaline forms were located at 525 nm and 625 nm which showed a relatively broader spectrum than other anthocyanins. An indicator film was fabricated by combining Roselle anthocyanin with agar and polyvinyl alcohol (PVA), which showed visible changes from red to green when employed to monitor the freshness of salmon stored at 4 °C. The ΔE value of Roselle anthocyanin indicator film was changed from 5.94 to >10. The ΔE value also can predict the chemical quality indicators of salmon effectively, especially with characteristic volatile components, and the predictive correlation coefficient was above 0.98. Therefore, the proposed indicating film showed great potential monitoring salmon freshness.

Keywords: Anthocyanin from different plant sources; Intelligent packaging based on colorimetric indicator; Salmon freshness.

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Conflict of interest statement

Declaration of competing interest We declare that we have no financial and personal relationships with other people or organizations that can inappropriately influence our work, there is no professional or other personal interest of any nature or kind in any product, service and or company that could be construed as influencing the position presented in, or the review of, the manuscript entitled.

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