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. 2023 Mar 30;20(1):57.
doi: 10.1186/s12985-023-02011-4.

Evaluation of ten (10) SARS-CoV-2 rapid serological tests in comparison with WANTAI SARS-CoV-2 ab ELISA in Burkina Faso, West Africa

Henri Gautier Ouedraogo  1 , Abdou Azaque Zoure  2 , Tegwinde Rebeca Compaoré  2 , Herve Ky  3   4 , Sylvie Zida  2 , Dezemon Zingué  5 , Oumarou Ouedraogo  2 , Serge Théophile Soubeiga  2 , Tani Sagna  2 , Charlemagne Dabiré  2 , Dinanibè Kambiré  2 , Dramane Zongo  2 , Albert Théophane Yonli  6 , Abdoul Rahamani Nikiema  2 , Désiré Nezien  7 , Gnintassa Cyrille Bansé  8 , Brice Wilfried Bicaba  9 , Sophie Perier  10 , Charles Sawadogo  4 , Zakariya Yabre  4 , Lassana Sangare  3
Affiliations

Evaluation of ten (10) SARS-CoV-2 rapid serological tests in comparison with WANTAI SARS-CoV-2 ab ELISA in Burkina Faso, West Africa

Henri Gautier Ouedraogo et al. Virol J. .

Abstract

Background: The aim of this study was to evaluate the performance of ten (10) SARS-CoV-2 serological rapid diagnostic tests in comparison with the WANTAI SARS-CoV-2 Ab ELISA test in a laboratory setting.

Materials and methods: Ten (10) SARS-CoV-2 serological rapid diagnostic tests (RDTs) for SARS-CoV-2 IgG/IgM were evaluated with two (2) groups of plasma tested positive for one and negative for the other with the WANTAI SARS-CoV-2 Ab ELISA. The diagnostic performance of the SARS-CoV-2 serological RDTs and their agreement with the reference test were calculated with their 95% confidence intervals.

Results: The sensitivity of serological RDTs ranged from 27.39 to 61.67% and the specificity from 93.33 to 100% compared to WANTAI SARS-CoV-2 Ab ELISA test. Of all the tests, two tests (STANDARD Q COVID-19 IgM/IgG Combo SD BIOSENSOR and COVID-19 IgG/IgM Rapid Test (Zhejiang Orient Gene Biotech Co., Ltd)) had a sensitivity greater than 50%. In addition, all ten tests had specificity greater than or equal to 93.33% each. The concordance between RDTs and WANTAI SARS-CoV-2 Ab ELISA test ranged from 0.25 to 0.61.

Conclusion: The SARS-CoV-2 serological RDTs evaluated show low and variable sensitivities compared to the WANTAI SARS-CoV-2 Ab ELISA test, with however a good specificity. These finding may have implications for the interpretation and comparison of COVID-19 seroprevalence studies depending on the type of test used.

Keywords: Antibody; COVID-19; ELISA; Point-of-care; Rapid serological diagnostic test; SARS-CoV-2.

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Conflict of interest statement

The authors declare that there are no conflicts of interests.

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