Sex differences in the expression of aggressive behavior and influences on social choice in zebrafish (Danio rerio)

Abstract

Aggregation behavior (grouping) provides many animals with the ecological benefits of protection from predators, increased access to food, and greater mating opportunities, despite possible costs. Many factors likely contribute to the social choices animals make, and we sought to determine if the individual expression of aggression would correlate with shoalmate choices. We quantified aggressive or submissive tendencies of individual male and female zebrafish and shoalmate preferences within dichotomous choice assays. We hypothesized that fish, regardless of their individual aggressive behaviors, would benefit most and therefore choose to spend time near larger shoals and shoals of the opposite sex. Both sexes spent significantly more time near shoals rather than be alone. Males spent significantly more time with the largest shoal, and females exhibited a similar trend. Both sexes spent more time with shoals of females, rather than males. Males were generally more consistent in their aggressive behaviors across multiple assays and females exhibited more individual-variability. More aggressive male zebrafish were more likely to choose shoals of males over females and were more likely to swim alone, but female zebrafish did not exhibit a correlation between aggressive behaviors and social choices. Our results demonstrate clear sex differences in the expression of individual behavior and influences on shoaling.

Keywords: Danio rerio; Sex differences; Shoaling; Zebrafish.