Separation of Cryptosporidium species oocysts from feces by using a percoll discontinuous density gradient
- PMID: 3700604
- PMCID: PMC268604
- DOI: 10.1128/jcm.23.1.199-200.1986
Separation of Cryptosporidium species oocysts from feces by using a percoll discontinuous density gradient
Abstract
Cryptosporidium oocysts were concentrated by an ether-phosphate-buffered saline sedimentation technique and then separated by density gradient centrifugation. This two-step method yielded highly concentrated oocysts largely free of bacteria and fecal debris.
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