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. 1986 Apr;23(4):800-2.
doi: 10.1128/jcm.23.4.800-802.1986.

Enhanced isolation of respiratory syncytial virus in cell culture

M Q Arens, E M Swierkosz, R R Schmidt, T Armstrong, K A Rivetna

Enhanced isolation of respiratory syncytial virus in cell culture

M Q Arens et al. J Clin Microbiol. 1986 Apr.

Abstract

During two winter seasons, we found that the combination of WI-38 or MRC-5 human lung fibroblasts plus primary rhesus monkey kidney (RhMK) and HEp-2 cell cultures yielded maximal isolation of respiratory syncytial virus. Cytopathic effects (CPE) developed earliest in RhMK cells and slowest in the human fibroblast lines. In RhMK cells, 50% of ultimately positive cultures showed CPE in 5 days, and 90% of positive cultures showed CPE within 7 days during both respiratory syncytial virus seasons.

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