Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Mar 27:15:149-161.
doi: 10.2147/JEP.S402277. eCollection 2023.

Pharmacological Evaluation of the Anesthetic and Analgesic Potential of Injection Harsha 22: A Novel Polyherbal Local Anesthetic Formulation Intended for Parenteral Administration in Wistar Albino Rats

Shan Sasidharan  1   2 , Asha Nair Kaveri  3 , M S Sithara  2 , Hareendran Nair J  4
Affiliations

Pharmacological Evaluation of the Anesthetic and Analgesic Potential of Injection Harsha 22: A Novel Polyherbal Local Anesthetic Formulation Intended for Parenteral Administration in Wistar Albino Rats

Shan Sasidharan et al. J Exp Pharmacol. .

Abstract

Background: Local anaesthetics are medications that cause numbness that can be reversed by applying them topically. Local anaesthetics are clinically used to control pain during minor surgeries or to treat other acute and chronic pain. The present investigation intended to investigate the anesthetic as well as analgesic potential of Injection Harsha 22, a novel polyherbal formulation in Wistar albino rats.

Methods: The anesthetic potential of Injection Harsha 22 was evaluated by a heat tail-flick latency (TFL) test, whereas the analgesic effect was elevated by electrical stimulation testing. Here, lignocaine (2%) was used as the standard anesthetic drug.

Results: In TFL, Injection Harsha 22 showed anesthetic effects up to 90 minutes after application. Also, the duration of anesthesia in rats that were administered subcutaneously with Injection Harsha 22 was comparable to that of the rats treated with commercial lignocaine (2%). In an electrical stimulation test, single administration of Injection Harsha 22 to rats significantly prolonged analgesia compared with the normal control group. The median duration of analgesia in rats administered subcutaneously with Injection Harsha 22 and lignocaine solution was 40 minutes and 35 minutes, respectively. Furthermore, Injection Harsha 22 does not interfere with the hematopoietic system of the experiment animals.

Conclusion: Thus, the present investigation established the in vivo anesthetic and analgesic potential of Injection Harsha 22 in experimental animals. Hence, it can be concluded that Injection Harsha 22 can become a prominent substitute for lignocaine as a local anaesthetic agent after establishing its efficacy through stringent clinical trials in humans.

Keywords: Wistar rats; analgesic; anesthetic; local anesthetic; polyherbal anesthetic formulation.

PubMed Disclaimer

Conflict of interest statement

The authors disclosed no conflicts of interest in this work.

Figures

Figure 1
Figure 1
The four anatomical sites selected for administering the Injection Harsha 22.
Figure 2
Figure 2
Tail flick latencies of rats after treatment with Injection Harsha 22 at different time intervals. Here Control: Normal saline, Test- Injection Harsha 22 and Standard- Lignocaine.
Figure 3
Figure 3
(A) Maximum possible effects - MPE (%) and (B) Area under the curve (AUC) of treatment groups at different time intervals. Here Control: Normal saline, Test- Injection Harsha 22 and Standard- Lignocaine.
Figure 4
Figure 4
Percentage (%) of animals with analgesia after treatment. Here Control: Normal saline, Test- Injection Harsha 22 and Standard- Lignocaine.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Abebe MM, Arefayne NR, Temesgen MM, Admass BA. Evidence-based perioperative pain management protocol for day case surgery in a resource limited setting: systematic review. Ann Med Surg. 2022;80:104322. doi:10.1016/j.amsu.2022.104322 - DOI - PMC - PubMed
    1. Yadav S, Jain S, Chaudhary J, Bansal R, Sharma M. The role of Ayurveda management in preventing surgical site infections instead of surgical antibiotic prophylaxis. J Ayurveda Integr Med. 2017;8(4):263–265. doi:10.1016/j.jaim.2017.03.003 - DOI - PMC - PubMed
    1. Sasidharan S, Kaveri Nair A, Sithara MS, Hareendran Nair J. A study on the acute dermal irritation of a novel polyherbal anesthetic formulation for parenteral administration in Wistar albino rats. Pharm Pharmacol Int J. 2022;10(5):168‒171. - PMC - PubMed
    1. Javahery S, Nekoubin H, Moradlu AH. Effect of anaesthesia with clove oil in fish (review). Fish Physiol Biochem. 2012;38(6):1545–1552. doi:10.1007/s10695-012-9682-5 - DOI - PubMed
    1. Zhang WK, Tao SS, Li TT, et al. Nutmeg oil alleviates chronic inflammatory pain through inhibition of COX-2 expression and substance P release in vivo. Food Nutr Res. 2016;60:30849. doi:10.3402/fnr.v60.30849 - DOI - PMC - PubMed